Effects of human pharmaceuticals on cytotoxicity, EROD activity and ROS production in fish hepatocytes. - Ineris - Institut national de l'environnement industriel et des risques Accéder directement au contenu
Article Dans Une Revue Toxicology Année : 2004

Effects of human pharmaceuticals on cytotoxicity, EROD activity and ROS production in fish hepatocytes.

Résumé

Pharmaceuticals are found in the aquatic environment but their potential effects on non-target species like fish remain unknown. This in vitro study is a first approach in the toxicity assessment of human drugs on fish. Nine pharmaceuticals were tested on two fish hepatocyte models: primary cultures of rainbow trout hepatocytes (PRTH) and PLHC-1 fish cell line. Cell viability, interaction with cytochrome P450 1A (CYP1A) enzyme and oxidative stress were assessed by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrasodium bromide tetrazolium (MTT), 7-ethoxyresorufin-o-deethylase (EROD) and dichlorofluorescein (DCFH-DA) assays, respectively. The tested drugs were clofibrate (CF), fenofibrate (FF), carbamazepine (CBZ), fluoxetine (FX), diclofenac (DiCF), propranolol (POH), sulfamethoxazole (SFX), amoxicillin (AMX) and gadolinium chloride (GdCl(3)). All substances were cytotoxic, except AMX at concentration up to 500 microM. The calculated MTT EC(50) values ranged from 2 microM (CF) to 651 microM (CBZ) in PLHC-1, and from 53 microM (FF) to 962 microM (GdCl(3)) in PRTH. CF, FF, and FX were the most cytotoxic drugs and induced oxidative stress before being cytotoxic. Compared to hepatocytes from human and dog, fish hepatocytes seemed to be more susceptible to the peroxisome proliferators (PPs) CF and FF. In PLHC-1 cells none of the tested drugs induced the EROD activity whereas POH appeared as a weak EROD inducer in PRTH. Moreover, in PRTH, SFX, DiCF, CBZ and to a lesser extend, FF and CF inhibited the basal EROD activity at clearly sublethal concentrations which may be of concern at the biological and chemical levels in a multipollution context.

Domaines

Toxicologie
Fichier principal
Vignette du fichier
Laville-etal-Toxicology2004.pdf (112.47 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

ineris-00167560 , version 1 (21-08-2007)

Identifiants

Citer

N. Laville, S. Aït-Aïssa, E. Gomez, C. Casellas, J. M. Porcher. Effects of human pharmaceuticals on cytotoxicity, EROD activity and ROS production in fish hepatocytes.. Toxicology, 2004, 196 (1-2), pp.41-55. ⟨10.1016/j.tox.2003.11.002⟩. ⟨ineris-00167560⟩
389 Consultations
1315 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More