Skip to Main content Skip to Navigation
Journal articles

Characterization of testicular expression of P450 17alpha-hydroxylase, 17,20-lyase in zebrafish and its perturbation by the pharmaceutical fungicide clotrimazole

Abstract : The aim of the present study was to characterize P450 17alpha-hydroxylase/17,20-lyase (cyp17a1) expression in zebrafish and to assess the effect of the pharmaceutical clotrimazole, a known inhibitor of various cytochrome P450 enzyme activities, on testicular gene and protein expression of this enzyme as well as on the testicular release of 11-ketotestosterone (11-KT), a potent androgen in fish. We first showed that cyp17a1 is predominantly expressed in gonads of zebrafish, notably in male. In vivo, clotrimazole induced a concentration-dependent increase of cyp17a1 gene expression and Cyp17-I protein synthesis in zebrafish testis. Using zebrafish testicular explants, we further showed that clotrimazole did not directly affect cyp17a1 expression but that it did inhibit 11-KT release. These novel data deserve further studies on the effect of azole fungicides on gonadal steroidogenesis.
Document type :
Journal articles
Complete list of metadatas

https://hal-ineris.archives-ouvertes.fr/ineris-00961766
Contributor : Gestionnaire Civs <>
Submitted on : Thursday, March 20, 2014 - 2:56:47 PM
Last modification on : Friday, May 29, 2020 - 12:08:09 AM
Long-term archiving on: : Friday, June 20, 2014 - 11:36:13 AM

File

2011-346_post-print.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Nathalie Hinfray, Damien Baudiffier, Marcello C. Leal, Jean-Marc Porcher, Selim Ait-Aissa, et al.. Characterization of testicular expression of P450 17alpha-hydroxylase, 17,20-lyase in zebrafish and its perturbation by the pharmaceutical fungicide clotrimazole. General and Comparative Endocrinology, Elsevier, 2011, 174 (3), pp.309-317. ⟨10.1016/j.ygcen.2011.09.008⟩. ⟨ineris-00961766⟩

Share

Metrics

Record views

371

Files downloads

680