Effect of prochloraz fungicide on biotransformation enzymes and oxidative stress parameters in three-spined stickleback (Gasterosteus aculeatus L.)

Wilfried Sanchez (1) , Benjamin Piccini (1) , Jean-Marc Porcher (1)

1 INERIS - Institut National de l'Environnement Industriel et des Risques

The aim of this study was to characterize biomarker responses in three-spined sticklebacks exposed to prochloraz (Pcz). For this purpose, adult sticklebacks were exposed for 2 weeks to prochloraz at 0, 10, 50, 100 and 500 mu g/L prior to one week of depuration in clean water. At days 7, 14 and 21, several hepatic biomarkers were measured including 7-ethoxyresorufin-O-deethylase (EROD), glutathione-S-transferase (GST), glutathione peroxidase (GPx), catalase (CAT), total glutathione (GSH) content and thiobarbituric acid reactive substances (TBARS). Pcz induced a transient increase of antioxidant enzymes and a depletion of glutathione content during the first 7 days of exposure. This study showed that EROD activity and antioxidants were disrupted in a transient manner. GST was rapidly induced in a dose-dependent manner and this induction was persistent and observed also after depuration. GST appeared as a valuable biomarker to assess the exposure to Pcz.

Keywords: BIOMARKERS; BIOTRANSFORMATION ENZYMES; LIVER; OXIDATIVE STRESS; PROCHLORAZ; THREE-SPINED STICKLEBACK

Domains: Ecotoxicology; Environmental Sciences

Wilfried Sanchez, Benjamin Piccini, Jean-Marc Porcher. Effect of prochloraz fungicide on biotransformation enzymes and oxidative stress parameters in three-spined stickleback (Gasterosteus aculeatus L.). Journal of Environmental Science and Health, Part B, 2008, 43 (1), pp.65-70. ⟨10.1080/03601230701735151⟩. ⟨ineris-00961921⟩