Cadmium induces apoptosis in a human T cell line

Abstract : Cadmium, a potent toxic metal, poses a serious environmental threat but the mechanisms of its toxicity remain unclear. In the present study, we investigated the nature of cadmium-induced cell death in the human T cell line CEM-C12. Cadmium was time- and dose-dependently toxic for CEM-C12 cells, cell death being preceded by chromatin condensation and DNA fragmentation. Quantification of the latter indicated an increase above 4 µM cadmium, with maximal fragmentation at 8 to 10 µM. By contrast, when CEM-C12 cells were exposed to higher cadmium concentrations (50 µM), cell death increased without concomitant chromatin condensation or DNA fragmentation. Thus, cadmium at low and high concentration kills CEM-C12 cells by apoptosis and necrosis, respectively. Addition of cycloheximide reduced the apoptotic effect of cadmium, suggesting that cadmium-induced apoptosis is an process depending on protein synthesis. Verapamil, a calcium/potassium channel blocker, markedly increased the viability of CEM-C12 cells treated by low cadmium concentrations and prevented DNA fragmentation. The apoptotic effect of cadmium suggests a possible mechanism for lymphocyte damage occurring after in vivo exposure to cadmium.
Document type :
Journal articles
Complete list of metadatas

https://hal-ineris.archives-ouvertes.fr/ineris-00962568
Contributor : Gestionnaire Civs <>
Submitted on : Friday, March 21, 2014 - 1:26:46 PM
Last modification on : Friday, March 21, 2014 - 1:26:46 PM

Identifiers

  • HAL Id : ineris-00962568, version 1
  • PUBMED : 8160194
  • INERIS : PU-1994-028

Collections

Citation

B. El Azzouzi, G.T. Tsangaris, O. Pellegrini, Y. Manuel, J. Benveniste, et al.. Cadmium induces apoptosis in a human T cell line. Toxicology, Elsevier, 1994, 88, pp.127-139. ⟨ineris-00962568⟩

Share

Metrics

Record views

94