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Journal Articles Toxicology Year : 1994

Cadmium induces apoptosis in a human T cell line

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1
B. El Azzouzi
  • Function : Author
G.T. Tsangaris
  • Function : Author
Y. Manuel
  • Function : Author
J. Benveniste
  • Function : Author
Y. Thomas
  • Function : Author

Abstract

Cadmium, a potent toxic metal, poses a serious environmental threat but the mechanisms of its toxicity remain unclear. In the present study, we investigated the nature of cadmium-induced cell death in the human T cell line CEM-C12. Cadmium was time- and dose-dependently toxic for CEM-C12 cells, cell death being preceded by chromatin condensation and DNA fragmentation. Quantification of the latter indicated an increase above 4 µM cadmium, with maximal fragmentation at 8 to 10 µM. By contrast, when CEM-C12 cells were exposed to higher cadmium concentrations (50 µM), cell death increased without concomitant chromatin condensation or DNA fragmentation. Thus, cadmium at low and high concentration kills CEM-C12 cells by apoptosis and necrosis, respectively. Addition of cycloheximide reduced the apoptotic effect of cadmium, suggesting that cadmium-induced apoptosis is an process depending on protein synthesis. Verapamil, a calcium/potassium channel blocker, markedly increased the viability of CEM-C12 cells treated by low cadmium concentrations and prevented DNA fragmentation. The apoptotic effect of cadmium suggests a possible mechanism for lymphocyte damage occurring after in vivo exposure to cadmium.
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ineris-00962568 , version 1 (21-03-2014)

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B. El Azzouzi, G.T. Tsangaris, O. Pellegrini, Y. Manuel, J. Benveniste, et al.. Cadmium induces apoptosis in a human T cell line. Toxicology, 1994, 88, pp.127-139. ⟨ineris-00962568⟩

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