Binding of serum autoantibodies to sialidase-treated tracheal epithelial cells. Determination of autoantibodies isotypes in normal and influenza virus infected guinea pig sera

Abstract : Cultured epithelial cells isolated from guinea pig trachea were treated with Vibrio cholerae sialidase. The treatment was not cytotoxic and resulted in membrane desialylation as assessed by measurement of sialic acids released, along with an increased fixation of the galactose-specific lectin peanut agglutinin. After incubation in serum from normal guinea pigs, membrane-bound immunoglobulins were detected using peroxidase-labelled antibodies. Sialidase-treated cells bound significantly more IgM than controls (P < 0.0005), whereas binding of IgG was not significantly different between treated and untreated cells (0.1 < P < 0.375); IgA were never detected. In influenza-infected guinea-pigs, as assessed by reactivity with peanut agglutinin, the tracheal and lung epithelium, as well as alveolar cells were hyposialylated. In these animals, the level of serum IgG autoantibodies capable to bind sialidase treated cultured cells increased, while the level of IgM autoantibodies did not change. These autoantibodies may participate in cellular dysfunctions and modified bronchoreactivity that occur during infection of the respiratory tract by sialidase-producing microorganisms, either through activation of the complement system, or subsequently to their reaction with cells expressing membrane complement and/or Fc receptors.
Document type :
Journal articles
Complete list of metadatas

https://hal-ineris.archives-ouvertes.fr/ineris-00962573
Contributor : Gestionnaire Civs <>
Submitted on : Friday, March 21, 2014 - 1:26:48 PM
Last modification on : Friday, March 21, 2014 - 1:26:48 PM

Links full text

Identifiers

Collections

Citation

M.A. Nahori, M. Etievant, H. Tzrzidistrabelsi, B. David, Claude Lambre. Binding of serum autoantibodies to sialidase-treated tracheal epithelial cells. Determination of autoantibodies isotypes in normal and influenza virus infected guinea pig sera. Immunology Letters, Elsevier, 1994, 42 (1-2), pp.67-73. ⟨10.1016/0165-2478(94)90037-X⟩. ⟨ineris-00962573⟩

Share

Metrics

Record views

93