Two-step purification method of vitellogenin from three teleost fish species : rainbow trout (Oncorhynchus mykiss), gudgeon (Gobio gobio) and chub (Leuciscus cephalus)

François Brion (1, 2) , Françoise Rogerieux (1) , Patrice Noury (2) , Bernard Migeon (2) , Patrick Flammarion (2) , Eric Thybaud (1) , Jean-Marc Porcher (1)

1 INERIS - Institut National de l'Environnement Industriel et des Risques
2 Laboratoire d'Ecotoxicologie

A two-step purification protocol was developed to purify rainbow trout (Oncorhynchus mykiss) vitellogenin (Vtg) and was successfully applied to Vtg of chub (Leuciscus cephalus) and gudgeon (Gobio gobio). Capture and intermediate purification were performed by anion-exchange chromatography on a Resource Q column and a polishing step was performed by gel permeation chromatography on Superdex 200 column. This method is a rapid two-step purification procedure that gave a pure solution of Vtg as assessed by silver staining electrophoresis and immunochemical characterisation

Mots clés: PURIFICATION; ONCORHYNCHUS MYKISS; GOBIO GOBIO; LEUCISCUS CEPHALUS; VITELLOGENIN

Domaines: Ecotoxicologie; Sciences de l'environnement

François Brion, Françoise Rogerieux, Patrice Noury, Bernard Migeon, Patrick Flammarion, et al.. Two-step purification method of vitellogenin from three teleost fish species : rainbow trout (Oncorhynchus mykiss), gudgeon (Gobio gobio) and chub (Leuciscus cephalus). Journal of Chromatography B - Analytical Technologies in the Biomedical and Life Sciences, 2000, 737 (1-2), pp.3-12. ⟨10.1016/S0378-4347(99)00406-5⟩. ⟨ineris-00962765⟩