Isolation, characterization and diuron transformation capacities of a bacterial strain arthrobacter sp N2

P. Widehem Selim Ait-Aissa 1 C. Tixier M. Sancelme H. Veschambre N. Truffaut
1 INERIS - Institut National de l'Environnement Industriel et des Risques
Abstract : A bacterial strain able to transform diuron was isolated from a soil by enrichment procedures. Strain isolation was realized by plating on minimal-agarose medium spread with this herbicide and selecting the colonies surrounded by a clear thin halo. One strain was characterized and identified as an Arthrobacter sp. It metabolized diuron and the final transformation product, 3,4-dichloroaniline, was produced in stoichiometric amounts. The transformation of diuron at different concentrations was more efficient in the presence of alternative sources of carbon and nitrogen. The bacterial activity was also evaluated in soil microcosms with a consequent disappearance of diuron and concomitant appearance of 3,4-dichloroaniline, of which the concentration decreased thereafter. Bacterial cells inoculated in the microcosms survived as viable but eventually nonculturable cells.
Keywords : ARTHROBACTER PHENYLUREA HERBICIDES DIURON BIODEGRADATION SOIL MICROCOSMS
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Journal articles
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Submitted on : Friday, March 21, 2014 - 1:48:56 PM
Last modification on : Friday, March 21, 2014 - 1:48:56 PM

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P. Widehem, Selim Ait-Aissa, C. Tixier, M. Sancelme, H. Veschambre, et al.. Isolation, characterization and diuron transformation capacities of a bacterial strain arthrobacter sp N2. Chemosphere, Elsevier, 2002, 46 (4), pp.527-534. ⟨10.1016/S0045-6535(01)00192-8⟩. ⟨ineris-00962808⟩

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