Assessment of estrogen (ER) and aryl hydrocarbon receptor (AhR) mediated activities in organic sediment extracts of the Detroit river, using in vitro bioassays based on human MELN and teleost PLHC-1 cell lines

Pascale Michallet-Ferrier 1 Selim Ait-Aissa 2 Patrick Balaguer 3 Janusz Dominik 1 G. Douglas Haffner 4 M. Pardos 1
1 Institute F.A. Forel
2 INERIS - Institut National de l'Environnement Industriel et des Risques
3 Endocrinologie moléculaire et cellulaire des cancers
4 Great Lakes Institute for Environmental Research / Department of Biological Sciences
Abstract : Binding to estrogen and aryl hydrocarbon receptors (ER and AhR) is a possible mechanism for a compound to mediate endocrine-disrupting effects. In the present study, substances contained in sediment organic fractions from the Detroit River were screened for their ability to bind ER and/or AhR. Two in vitro bioassays were used: MELN cells, consisting of human breast cancer cells (MCF-7) stably transfected with the luciferase reporter gene, to assess the binding to ER, and cultured fish hepatocytes, PLHC-1, to measure the AhR-mediated induction of 7-ethoxyresorufin-O-deethylase (EROD). Chemical analyses of the sediment extracts were performed to examine relationships with the biological responses. Significant ER- and AhR-activities were mostly found in the most polar fraction (containing mostly PAHs). The five strongest estrogenic extracts were also the most efficient to induce EROD activity. The correlation between PAHs contamination level and biological responses suggests that the most polluted areas contain a mixture of both estrogenic and EROD-inducing compounds or, alternatively, compounds able to bind ER and AhR, such as PAHs, would be responsible for the observed activities.
Mots-clés : SEDIMENT DETROIT RIVER ENDOCRINE DISRUPTION MELN PLHC-1 PAHS
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Journal articles
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https://hal-ineris.archives-ouvertes.fr/ineris-00962889
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Submitted on : Friday, March 21, 2014 - 1:59:43 PM
Last modification on : Friday, December 22, 2017 - 4:48:01 PM

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  • HAL Id : ineris-00962889, version 1
  • INERIS : PU-2004-023

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Pascale Michallet-Ferrier, Selim Ait-Aissa, Patrick Balaguer, Janusz Dominik, G. Douglas Haffner, et al.. Assessment of estrogen (ER) and aryl hydrocarbon receptor (AhR) mediated activities in organic sediment extracts of the Detroit river, using in vitro bioassays based on human MELN and teleost PLHC-1 cell lines. Journal of Great Lakes Research, Elsevier, 2004, 30 (1), pp.82-92. ⟨ineris-00962889⟩

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