Skip to Main content Skip to Navigation
Journal articles

Selecting a battery of bioassays for ecotoxicological characterization of wastes

Abstract : This study was conducted in France within the context of waste classification (Hazardous Waste Council Directive 91/689/ EEC), and focused on "ecotoxic" property (H14). In 1998, an experimental test strategy was developed to assess ecotoxicological properties of wastes using a battery of six standardized bioassays. This combined direct and indirect approaches integrating two solid-phase tests: emergence and growth inhibition of Lactuca sativa (14 days), mortality of Eiseniajetida (14 days) and four standardized tests performed on water extracts from wastes : growth inhibition of Pseudokirchneriella subcapitata (3 days), inhibition of mobility of Daphnia magna (48 h), inhibition of reproduction of Ceriodaphnia dubia (7 days), inhibition of light emission of Vibrio fischeri (30 min). This study aimed to set up preliminary conclusions on relevancy of this experimental test strategy, based on data obtained since 1998. Results were analyzed from the combined use of Hierarchical Cluster Analysis, Principal Component Analysis and Nonlinear Mapping. These multivariate analyses clearly showed that it was possible to reduce this number of tests without changing the typology of the wastes. A battery of bioassays including one solid phase test and two tests performed on water extracts (L. sativa, V. fischeri and C. dubia) was found as an optimal solution for characterizing the toxicity of the studied wastes. This optimal battery represents a good basis for determining the H14 property.
Document type :
Journal articles
Complete list of metadata
Contributor : Gestionnaire Civs Connect in order to contact the contributor
Submitted on : Friday, March 21, 2014 - 2:07:21 PM
Last modification on : Friday, February 26, 2021 - 3:20:02 PM

Links full text




Pascal Pandard, James Devillers, Anne-Marie Charissou, Véronique Poulsen, Marie-José Jourdain, et al.. Selecting a battery of bioassays for ecotoxicological characterization of wastes. Science of the Total Environment, Elsevier, 2006, 363 (1-3), pp.114-125. ⟨10.1016/j.scitotenv.2005.12.016⟩. ⟨ineris-00963022⟩



Record views