Longitudinal 3He and proton imaging of magnetite biodistribution in a rat model of instilled nanoparticles

Achraf Al Faraj 1 Ghislaine Lacroix 2 Hasan Alsaid 1 Dan Elgrabli 2 Vasile Stupar 1 Franck Robidel 2 Sophie Gaillard 1 Emmanuelle Canet-Soulas 1 Yannick Gremillieux 1
1 CREATIS - Centre de Recherche et d'Application en Traitement de l'Image et du Signal
2 INERIS - Institut National de l'Environnement Industriel et des Risques
Abstract : Epidemiological and toxicological studies have provided evidence that accidentally inhaled nanosize ultrafine particles can induce chronic or acute health damage. MRI, being noninvasive, is able to assess the biodistribution and clearance of magnetically labeled nanoparticles induced by instillation or inhalation. We report He-3 and proton MRI follow-up of lung, liver, spleen, and kidney distribution of USPIO (ultrasmall superparamagnetic iron oxide) in a rat model. The sensitivity of the imaging technique to various concentrations of instilled magnetite suspension was first assessed in vivo (n = 12). A 2-week longitudinal imaging study was then performed on animals (n = 7) instilled with a 0.5 mg magnetite solution. Hypointense and void signal regions associated with intrapulmonary USPIO were observed in the He-3 ventilation images throughout the study, whereas no USPIO-related proton signal intensity changes were found. Intrapulmonary magnetite nanoparticle confinement was confirmed by ex vivo iron assay and histological analysis. This study demonstrates that combined 3 He and proton MRI enables noninvasive assessment of the distribution and clearance of magnetically labeled instilled nanoparticles
Keywords : HYPERPOLARIZED HELIUM LUNG MRI HEALTH AND ENVIRONMENT IMPACT NANOPARTICLES NONINVASIVE ASSESSMENT
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Journal articles
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https://hal-ineris.archives-ouvertes.fr/ineris-00963121
Contributor : Gestionnaire Civs <>
Submitted on : Friday, March 21, 2014 - 2:12:43 PM
Last modification on : Wednesday, November 20, 2019 - 3:08:05 AM

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Achraf Al Faraj, Ghislaine Lacroix, Hasan Alsaid, Dan Elgrabli, Vasile Stupar, et al.. Longitudinal 3He and proton imaging of magnetite biodistribution in a rat model of instilled nanoparticles. Magnetic Resonance in Medicine, Wiley, 2008, 59 (6), pp.1298-1303. ⟨10.1002/mrm.21571⟩. ⟨ineris-00963121⟩

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