Effect of (xeno)-estrogens on zebrafish P450c17 (C17, 17 alpha-hydroxylase/17, 20-lyase) mRNA and protein expression in gonadal tissue

François Brion (1) , Nathalie Hinfray (1) , Olivier Palluel (1) , Julien Maillard (1) , Isabelle Anglade (2) , Selim Ait-Aissa (1) , Olivier Kah (2) , Jean-Marc Porcher (1)

1 INERIS - Institut National de l'Environnement Industriel et des Risques
2 ICM - Interactions cellulaires et moléculaires

The objectives of this study were to determine the expression and the cellular localization of P450c17 in gonadal tissues in the zebrafish and to investigate the effect of (xeno)-hormones on its expression at both mRNA and protein levels. We showed that gonads are sites for cyp17 gene expression and we documented for the first time the cellular localization of the cyp17 protein expression within ovarian and testicular tissues in a cyprinid fish. Moreover, we demonstrated the down-regulating effect of (xeno)-estrogens on testicular cyp17 expression.

Mots clés: STEROIDOGENESE; P450C17; GENE PROTEINE EXPRESSSION; GONADES; POISSON ZEBRE<br />---<br />STEROIDOGENESIS; GENE EXPRESSION; GONADS; ZEBRAFISH

Domaines: Ecotoxicologie; Sciences de l'environnement

François Brion, Nathalie Hinfray, Olivier Palluel, Julien Maillard, Isabelle Anglade, et al.. Effect of (xeno)-estrogens on zebrafish P450c17 (C17, 17 alpha-hydroxylase/17, 20-lyase) mRNA and protein expression in gonadal tissue. Cybium : Revue Internationale d’Ichtyologie, 2008, 32 (2, suppl. S), pp.245-246. ⟨ineris-00963150⟩