Skip to Main content Skip to Navigation
Journal articles

A standardized method for the sampling of rhizosphere and rhizoplan soil bacteria associated to a herbaceous root system

Abstract : Plants-microorganisms interactions play a fundamental role in terrestrial ecosystems and various methods have been reported for plant-associated bacteria extraction. However, these methods exhibit notable variations and lack of some procedural details that may impact the interpretations of results. We propose here a standardized and detailed protocol for the independent extraction of bulk, rhizosphere and rhizoplan soil fractions. This protocol was applied to the sampling of different polluted soil fractions collected in the vicinity of Arabidopsis halleri dense root system. It allowed us to determine the cultivable bacterial densities in each fraction and to confirm the existence of a bacterial gradient linked to roots distance, with a higher amount of bacteria in the rhizospheric area. We suggest to use this unified procedure as a common basis for soil sampling and bacterial communities analysis from other roots systems.
Document type :
Journal articles
Complete list of metadatas

https://hal-ineris.archives-ouvertes.fr/ineris-00963444
Contributor : Gestionnaire Civs <>
Submitted on : Friday, March 21, 2014 - 2:31:46 PM
Last modification on : Wednesday, September 5, 2018 - 2:50:09 PM

Identifiers

Collections

Citation

Cindy Barillot, Claude Sarde, Valérie Bert, Eric Tarnaud, Nelly Cochet. A standardized method for the sampling of rhizosphere and rhizoplan soil bacteria associated to a herbaceous root system. Annals of Microbiology, Springer, 2013, 63 (2), pp.471-476. ⟨10.1007/s13213-012-0491-y⟩. ⟨ineris-00963444⟩

Share

Metrics

Record views

215