Daphnia tests
Résumé
The proposed test strategy combined the direct and indirect approaches, integrating organisms representing the terrestrial and aquatic ecosystems. For the latter, several tests were selected; the Daphnia magna mobility test, the algal growth inhibition test and the inhibition of light emission of Vibrio fischeri were considered as a basic test set. From a methodological point of view, no significant problems were reported during the ring test. A total of 149 tests out of the 161 tests conducted were considered acceptable. The overall results showed that the D. magna mobility test was highly sensitive to incineration ash and contaminated wood eluates. On the other hand, high variability in the results was observed mainly for the contaminated wood eluate (INC, min-max: 10; WOO, min-max: 31). The CVs were 62 and 103% for INC and WOO respectively. In contrast to the terrestrial tests, the variability of the results shows not only the variability of the ecotoxicity test itself, but also integrates the variability of the overall procedure: sampling of the test portion, eluate preparation (leaching procedure, separation of the solid and liquid phases) and the ecotoxicity test. It may partly explain the high variability of the results of the waste tests compared with those observed for tests with the reference chemical.