Ceriodaphnia dubia Chronic Toxicity Tests

C. Bazin , Pascal Pandard (1) , Anne-Marie Charissou (2) , Y. Barthel

1 INERIS - Institut National de l'Environnement Industriel et des Risques
2 Laboratoire Etudes et Expertises

Crustaceans are considered an important part of the soft water zooplankton community. Therefore they represent water organisms in the test set for the ecotoxicological characterization of wastes. Following ISO/CD guideline 20665, the chronic Ceriodaphnia dubia test was performed with three waste types: INC, SOI, and WOO. All the ten tests performed were classified as acceptable. Methodologically, almost no problems occurred. The min-max factors (2.08-2.54) between individual EC50 values and the CVs (31-33%) were low for waste eluates, showing that the chronic Ceriodaphnia dubia test is a robust method. These results are in agreement with the few data on substances known from the literature. The obtained data show that the test is able to discriminate different toxicity levels: INC = 4.8%, SOI > 90%, WOO = 0.08%. The sensitivity of the Ceriodaphnia chronic test is equivalent to the rotifer chronic test and slightly higher than the Algae test for the wood eluate. Therefore it is recommended for the toxicity assessment of waste.

Keywords: CERIODAPHNIA DUBIA; CHRONIC TEST; REPRODUCTION; RING TEST; ELUATE; WASTE

Domains: Life Sciences [q-bio]; Toxicology; Ecotoxicology; Environmental Sciences

C. Bazin, Pascal Pandard, Anne-Marie Charissou, Y. Barthel. Ceriodaphnia dubia Chronic Toxicity Tests. MOSER, Heidrun ; ROMBKE, Jorg. Ecotoxicological Characterization of Waste, Springer. New York, pp.161-167, 2009, Earth and Environmental Science. ⟨ineris-00969411⟩