HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Conference papers

Pulmonary effects of exposure to acetaldehyde or benzaldehyde in ovalbumin-sensitized Guinea-pigs

Abstract : The pulmonary effects of two environmentally relevant aldehydes were investigated in ovalbumin (OA)-sensitized guinea-pigs (GP). Four week-old male Hartley GP, weighing about 300 g, were intraperitoneally injected with 1 ml of a NaCl solution containing 100 µg OA and 100 mg Al(OH)3. They were then exposed either to acetaldehyde or benzaldehyde (500 ppb each) during 4 weeks (6 hours/day, 5 days/week). At the end of exposure, GP were challenged with an OA aerosol (0.1% in NaCl) and pulmonary functions were measured. The day after, guinea-pigs were anesthetized and several endpoints related to inflammatory and allergic responses were assessed in : blood (numeration, formula, biochemistry), whole lung (histology), and bronchoalveolar lavage (BAL) (differential cell count, protein and eicosanoid concentrations). Sensitized non-exposed GP (n = 8) had an increased number of eosinophils in blood and BAL, together with an increase in total protein and leukotrien (LTB4 and LTC4/D4/E4) contents in BAL. In non-sensitized GP (n = 8), exposure to acetaldehyde or benzaldehyde did not induce any change in the tested parameters, with the exception of a slight irritation of the respiratory tract as detected by histology and an increased number of alveolar macrophages in animals exposed to acetaldehyde (21.9 +- 1.6 and 42.2 +- 5.4 x 106 cells in control and exposed GP respectively). In sensitized GP, exposure to acetaldehyde induced a moderate irritation of the respiratory tract but no change in biological parameters linked to the inflammatory and allergic responses. In contrast, exposure to benzaldehyde induced : a decrease in both OA-induced bronchoconstriction and neutrophil number in BAL, an increase in the bronchodilatator mediator PGE2 and a decrease in the bronchoconstrictor mediators LTC4/D/E4. Further investigations are needed to determine if the attenuated response observed in sensitized GP exposed to benzaldehyde is due to an alteration of the mechanism of sensitization or to a more direct effect on various mechanisms of the allergic response.
Document type :
Conference papers
Complete list of metadata

Contributor : Gestionnaire Civs Connect in order to contact the contributor
Submitted on : Wednesday, April 2, 2014 - 3:31:50 PM
Last modification on : Monday, March 14, 2022 - 3:36:03 PM


  • HAL Id : ineris-00969746, version 1
  • INERIS : PU-2000-043



Ghislaine Lacroix, Sylvie Tissot, Françoise Rogerieux, Franck Robidel, Line Cornu, et al.. Pulmonary effects of exposure to acetaldehyde or benzaldehyde in ovalbumin-sensitized Guinea-pigs. 38. European Congress of Toxicology (EUROTOX 2000), Sep 2000, Londres, United Kingdom. ⟨ineris-00969746⟩



Record views