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Kinetics of pulmonary inflammatory response following exposure to ozone or nitrogen dioxide

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Abstract

The kinetics of pulmonary inflammatory response were investigated in male Balb/c mice (25-27 g) exposed to 1 ppm O3 or 50 ppm NO2 for 3h. At various times after beginning of exposure (3 /24 /48 / 72/ 96 /120 h), airway responsiveness to metacholine was assessed using whole-body plethysmography. Animals were then anesthetized and several inflammatory mediators (proteins, LDH, cytokines, eicosanoids) were measured in the bronchoalveolar lavage (BAL). Alveolar and peritoneal macrophages were tested for phagocytosis. Proliferative response of splenocytes was also evaluated. Exposed animals had mainly effects at the pulmonary level, except for a decrease in thymus weight at 24 to 72h after exposure to NO2 and at 48h after exposure to O3. Mice exposed to NO2 showed airway hyperresponsiveness till 72h post exposure. Response to ozone was mild and transient with bronchial hyperreactivity only just after exposure. NO2 exposure induced an important pulmonary inflammatory response characterized by a neutrophil influx at 48h, a persistent alteration of epithelial permeability and a transient increase in LDH levels in BAL. TNF-, PGF2 and IL-13 were unchanged in BAL. IL-6 and LTB4 levels temporarily increased just after exposure, IL-1 level peaked at 48h and persistent increased levels of PGE2 and LTC4/D4/E4 were noted. AM phagocytosis was also decreased between 3 and 48h after NO2 exposure. Ozone-induced inflammatory response was less intense, with only a neutrophil influx after 48h and a transient increase in IL-6 level in BAL just after the end of exposure. This study is the first step in studying the effects of exposure to a combination of these two gases.
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ineris-00969760 , version 1 (02-04-2014)

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Ghislaine Lacroix, D. Rome, Françoise Rogerieux, Franck Robidel, Barbara Proust, et al.. Kinetics of pulmonary inflammatory response following exposure to ozone or nitrogen dioxide. ATS 2001, May 2001, San Francisco, United States. ⟨ineris-00969760⟩

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