Seasonal variations of dioxine-like activity in sediments of the Bizerta lagoon (Tunisia) detected with in vitro cellular bioassays

I. Louiz 1 O.K. Ben-Hassine 1 M. Ben-Attia 2 Saïd Kinani 3 Selim Ait-Aissa 3
1 Unité de recherche Biologie, Ecologie et Parasitologie des Organisme Aquatiques
2 DCMR - Laboratoire des mécanismes réactionnels
3 INERIS - Institut National de l'Environnement Industriel et des Risques
Abstract : In order to evaluate sediment contamination in six stations from Bizerta lagoon, a combination of in vitro bioassays and chemical analysis was applied to sediment organic extracts. By using 7- ethoxyresorufin-O-deethylase (EROD) induction in the fish hepatoma cell line PLHC-1 after 4 h of cell exposure, dioxin-like activities were detected in all analysed samples. Dioxin-like activities were higher in winter than in summer. A highly significant correlation was observed between bioassay- and chemical analyses-derived toxic equivalents (TEQs), but PAHs accounted for only a small part of the detected biological activities. Our results suggest the presence of unknown readily metabolised EROD inducing compounds, which will need to be further characterized
Keywords : LAGOONS POLLUTION SEDIMENTS ECOTOXICOLOGY
Document type :
Conference papers
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Submitted on : Wednesday, April 2, 2014 - 3:52:31 PM
Last modification on : Wednesday, March 20, 2019 - 12:12:02 PM

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  • HAL Id : ineris-00970659, version 1
  • INERIS : EN-2010-218

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INERIS | X | X-DCMR | PARISTECH | X-DEP-CHIM

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I. Louiz, O.K. Ben-Hassine, M. Ben-Attia, Saïd Kinani, Selim Ait-Aissa. Seasonal variations of dioxine-like activity in sediments of the Bizerta lagoon (Tunisia) detected with in vitro cellular bioassays. 39. CIESM Congress, May 2010, Venise, Italy. ⟨ineris-00970659⟩

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