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Optimization of accelerated solvant extraction (ASE) of wide range of endocrine disrupting chemicals (EDC) in sediment

Abstract : Aquatic sediments are the sink for thousands of chemical pollutants. Among them, EDCs encompass a wide diversity of chemical classes from natural and anthropogenic origins, acting on various signaling pathways, and the use of in vitro bioassays is now widely accepted as an integrative method for their detection in complex samples. However, the diversity of EDC chemical properties makes difficult their common extraction and validation of extraction method for bioanalysis is still weakly documented. Hence, the aim of this study was to optimize and validate a method allowing a rapid and efficient extraction of a broad range of active chemicals. A mixture of 12 EDCs (i.e. estrone, 17beta-estradiol, bisphenol A, o,pDDT, 4-tert-octylphenol, fenofibrate, triphenyl phosphate, clotrimazole, PCB-126, 2,3,7,8 TCDD, Benzo[k]fluoranthene and dibenzo[a,h]anthracene) with wide range of polarity (2 80 %) whereas the heptane was the less efficient (50-60 %). First results obtained with chemical analyses were in accordance with bioassay data. Finally, similar results were obtained in natural sediment. In conclusion, this study reports a comprehensive assessment of extraction conditions before application in different in vitro bioassays, which has been rarely studied. Although there was no marked differences between extraction solvant, mixture of polar and non-polar solvent were shown to offer the best extraction of a wide range of active EDCs.
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Submitted on : Wednesday, April 2, 2014 - 3:52:44 PM
Last modification on : Tuesday, December 7, 2021 - 1:52:03 PM


  • HAL Id : ineris-00970781, version 1
  • INERIS : EN-2011-107



Nicolas Creusot, Marie-Hélène Devier, Jean-Marc Porcher, Hélène Budzinski, Selim Ait-Aissa. Optimization of accelerated solvant extraction (ASE) of wide range of endocrine disrupting chemicals (EDC) in sediment. 21. SETAC Europe annual meeting "Ecosystem protection in a sustainable world : a challenge for science and regulation", May 2011, Milan, Italy. ⟨ineris-00970781⟩



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