Sediment contact test with Myriophyllum aquaticum (ISO/DIS 16191) : results of an international ring test

Abstract : Although they are an important part of an aquatic ecosystem, dicotyledonous macrophytes are not yet part of the risk assessment of sediments or dredged material. A sediment contact test system with the dicotyledonous watermilfoil Myriophyllum aquaticum was established by the ecotoxicological laboratory of the German Federal Institute of Hydrology. This test procedure is now in the standardisation process within ISO. In the sediment contact test, Myriophyllum aquaticum whorls are exposed to environmental samples for ten days. The growth of Myriophyllum aquaticum in a test sample is compared with its growth in the control sample. Phytotoxic effects can be quantified as growth inhibition (%) relative to the control growth. Several laboratories from academia, industries and government participated in an interlaboratory ring test organized by the German Federal Institute of Hydrology. The ring test aimed at i) investigating the practicability and reproducibility of the sediment contact test, ii) validating the chosen endpoint, and iii) determining the response range of the reference substance functioning as positive control.