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In-vivo experimental model for silicosis : fisher 344, sprague dawley and wistar rat strains

Abstract : The selection of an experimental model for silicosis requires a thorough understanding of a number of different pulmonary parameters specific to the animal that is to be used (e.g., clearance time, penetration curves, anatomical differences...), and of the sensitivity of the strains that are to be used. The pulmonary response of three rat strains (i.e., Fisher 344, Sprague Dawley, Wistar) to silica dust were compared using two different exposure methods: intra-tracheal injections and inhalation. The test periods lasted 3 months for injection and 6 and 12 months for inhalation. The histological study of the lung revealed a distinct nodular reaction among Sprague Dawley and Wistar strains. Intra-tracheal injections led to the development of fibrotic nodules among Wistar rats, whereas such silicotic nodules were infrequent among injected Fisher 344 rats, and almost absent when exposure was by inhalation. Sprague Dawley and Fisher 344 rats showed frequent thickening and metaplasia of the alveolar walls near the terminal bronchioles. This tendency was particularly pronounced among rats exposed by inhalation (especially among Fisher 344 rats). Evaluation of wet lung weight (i.e., hydroxyproline, lipid and silica lung contents) reveals an increase in the different parameters for Sprague Dawley rats relative to the other two strains, regardless of the type of exposure. It has thus been concluded that Wistar rats are the best experimental model for silicosis, as their pulmonary reaction is more characteristic than that of the other two strains.
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  • HAL Id : ineris-00971884, version 1
  • INERIS : PU-1993-079



Sylvie Honnons, Dominique Oberson, Jean-Marc Porcher. In-vivo experimental model for silicosis : fisher 344, sprague dawley and wistar rat strains. 2. International Symposium on Silica Silicosis & Cancer, Oct 1993, San Francisco, United States. ⟨ineris-00971884⟩



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