Brain aromatase (Cyp19a1b) is a highly sensitive gene to estrogens and xeno-estrogens

Abstract : Aromatase is the only enzyme responsible for the irreversible conversion of androgens into estrogens. Teleost fishes have two copies of the cyp19a1 gene that encode two isoforms of aromatase: cyp19a1a encodes ovarian aromatase, while the cyp19a1b gene encodes brain aromatase (aromatase B). We have shown that (i) aromatase B is strongly expressed in radial glial cells (RGC), that act as stem cells in mammals and fish and ii) the cyp19a1b gene is very sensitive to estrogens, through a mechanism that involves a well conserved ERE. This feature makes this gene an outstanding biomarker of xeno-estrogen exposures and we have developed and validated an in vivo assay allowing detection of estrogenic activity with a very high sensitivity. The in vivo assay is based on a transgenic zebrafish tg(cyp19a1b-GFP) line that expresses GFP in RGCs and we demonstrate the usefulness of the transgenic cyp19a1b-GFP as a reliable, sensitive and rapid in vivo estrogenic screening assay.
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  • HAL Id : ineris-00973652, version 1
  • INERIS : EN-2012-005

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François Brion, Yann Le Page, Benjamin Piccini, Sok-Keng Tong, Bon-Chu Chung, et al.. Brain aromatase (Cyp19a1b) is a highly sensitive gene to estrogens and xeno-estrogens. Endocrine Disruptors 2012, Feb 2012, Munich, Germany. pp.NC. ⟨ineris-00973652⟩

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