Brain aromatase (Cyp19a1b) is a highly sensitive gene to estrogens and xeno-estrogens - Archive ouverte HAL Access content directly
Conference Papers Year : 2012

Brain aromatase (Cyp19a1b) is a highly sensitive gene to estrogens and xeno-estrogens

(1) , (2) , (1) , (3) , (3) , (2)
1
2
3

Abstract

Aromatase is the only enzyme responsible for the irreversible conversion of androgens into estrogens. Teleost fishes have two copies of the cyp19a1 gene that encode two isoforms of aromatase: cyp19a1a encodes ovarian aromatase, while the cyp19a1b gene encodes brain aromatase (aromatase B). We have shown that (i) aromatase B is strongly expressed in radial glial cells (RGC), that act as stem cells in mammals and fish and ii) the cyp19a1b gene is very sensitive to estrogens, through a mechanism that involves a well conserved ERE. This feature makes this gene an outstanding biomarker of xeno-estrogen exposures and we have developed and validated an in vivo assay allowing detection of estrogenic activity with a very high sensitivity. The in vivo assay is based on a transgenic zebrafish tg(cyp19a1b-GFP) line that expresses GFP in RGCs and we demonstrate the usefulness of the transgenic cyp19a1b-GFP as a reliable, sensitive and rapid in vivo estrogenic screening assay.
Fichier principal
Vignette du fichier
2012-005_hal.pdf (355.25 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

ineris-00973652 , version 1 (04-04-2014)

Identifiers

Cite

François Brion, Yann Le Page, Benjamin Piccini, Sok-Keng Tong, Bon-Chu Chung, et al.. Brain aromatase (Cyp19a1b) is a highly sensitive gene to estrogens and xeno-estrogens. Endocrine Disruptors 2012, Feb 2012, Munich, Germany. pp.NC. ⟨ineris-00973652⟩
389 View
315 Download

Share

Gmail Facebook Twitter LinkedIn More