Genotoxic effects of ultraviolet (UV) radiation on human 3D skin model in vitro

Abstract : The aim of this experiment was to examine whether the UVA or UVB exposure with two doses each has genotoxic effect on human reconstructed skin in vitro. The DNA damage was detected by comet assay. In this preliminary study we managed to show the direct damaging effect of the UVB radiation on the DNA, but UVA exposure did not have any significant effect. This experiment was done as a preliminary study for the project named “Cellular response to co-exposure of radiofrequency (RF) and solar ultraviolet (UV) radiation in human in vitro skin model (SKIN-RF)”.