Effects of ultraviolet radiation on full thickness human skin model in vitro (SKIN-RF project)

Abstract : The aim of this study was to examine whether the solar ultraviolet (UV) radiation has any effect on cytokine (IL-1α, IL-6, IL-8) and MMP-1 enzyme secretion on human skin model in vitro. The full thickness skin models were exposed by a sun simulator lamp to UV radiation (1.5 SED, 3 SED). The IL-1α, IL-6, IL-8 and MMP-1 concentrations were measured 24 hours after UV exposure from the culture medium by the ELISA method. In this study we managed to show that UV radiation doses of 1.5 SED, 3 SED did not alter the level of these factors. This experiment was done as a preliminary study for the project named “Cellular response to co-exposure of radiofrequency (RF) and solar ultraviolet (UV) radiation in human in vitro skin model (SKIN-RF)”.