A bacterium-based contact assay for evaluating the quality of solid samples. Results from an international ring-test.

Catarina R. Marques Najoi El-Azhari Fabrice Martin-Laurent Pascal Pandard 1 Camille Meline 1 Alice L. Petre 2 Suzanne Eckert Jurgen Zipperle Martin Vana 3 Stanislav Maly 3 Lucie Sindelarova 4 Anna Slavikova Amemori 4 Jakub Hofman 4 Anu Kumar Hai Doan Mike Mclaughlin Elizabeth Richter 5 Jörg Römbke
1 INERIS - Institut National de l'Environnement Industriel et des Risques
2 University of Alcalá / Department of Analytical Chemistry, Physical-Chemistry and Chemical
3 Central Institute for Supervising and Testing in Agriculture
4 RECETOX - Research Centre for Toxic Compounds in the Environment
5 Goethe-University Frankfurt am Main
Abstract : The contact assay measuring the inhibition of Arthrobacter globiformis dehydrogenase activity as an endpoint to evaluate the toxicity of solid samples was tested in an international ring-test to validate its performance for ISO standardization (ISO/CD 18187). This work reports the results of the ring-test involving 9 laboratories from six countries. At least 8 valid data sets were obtained for each sample and more than three quarters of the participants attained the validity criteria defined in the standard. The coefficient of variation within (CVr) and between (CVR) laboratories was generally on average <15% and <30% for negative and positive controls, respectively. Regarding solid samples, the laboratories provided a similar ranking of the samples based on their toxicity, despite some variation in the LOEC values. The logarithmic within-lab standard deviation <0.50 for soils and <0.25 for wastes evidenced a good repeatability. The between-lab variability assessed by a CVR <30%, minimum-maximum factor <4 and a reproducibility standard deviation (SDR) <0.13 for a great part of the solid samples, confirmed the test reproducibility. Overall, this assay proved to be robust, sensitive and feasible for routine use towards the quality assessment of soils and wastes.
Keywords : ARTHROBACTER GLOBIFORMIS DEHYDROGENASE ACTIVITY SOILS WASTES ISO 18187
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Journal articles
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https://hal-ineris.archives-ouvertes.fr/ineris-01853329
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Submitted on : Friday, August 3, 2018 - 10:13:18 AM
Last modification on : Thursday, October 17, 2019 - 12:04:02 PM

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Catarina R. Marques, Najoi El-Azhari, Fabrice Martin-Laurent, Pascal Pandard, Camille Meline, et al.. A bacterium-based contact assay for evaluating the quality of solid samples. Results from an international ring-test.. Journal of Hazardous Materials, Elsevier, 2018, 352, pp.139-147. ⟨10.1016/j.jhazmat.2018.03.022⟩. ⟨ineris-01853329⟩

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