Service interruption on Monday 11 July from 12:30 to 13:00: all the sites of the CCSD (HAL, EpiSciences, SciencesConf, AureHAL) will be inaccessible (network hardware connection).
Skip to Main content Skip to Navigation
Journal articles

The comet assay in higher terrestrial plant model: Review and evolutionary trends

Abstract : The comet assay is a sensitive technique for the measurement of DNA damage in individual cells. Although it has been primarily applied to animal cells, its adaptation to higher plant tissues significantly extends the utility of plants for environmental genotoxicity research. The present review focuses on 101 key publications and discusses protocols and evolutionary trends specific to higher plants. General consensus validates the use of the percentage of DNA found in the tail, the alkaline version of the test and root study. The comet protocol has proved its effectiveness and its adaptability for cultivated plant models. Its transposition in wild plants thus appears as a logical evolution. However, certain aspects of the protocol can be improved, namely through the systematic use of positive controls and increasing the number of nuclei read. These optimizations will permit the increase in the performance of this test, namely when interpreting mechanistic and physiological phenomena.
Document type :
Journal articles
Complete list of metadata
Contributor : Gestionnaire Civs Connect in order to contact the contributor
Submitted on : Monday, August 6, 2018 - 2:53:57 PM
Last modification on : Wednesday, March 23, 2022 - 3:51:06 PM




Caroline Lanier, Nicolas Manier, Damien Cuny, Annabelle Deram. The comet assay in higher terrestrial plant model: Review and evolutionary trends. Environmental Pollution, Elsevier, 2015, 207, pp.6-20. ⟨10.1016/j.envpol.2015.08.020⟩. ⟨ineris-01854103⟩



Record views