Juvenile roach (Rutilus rutilus) increase their anaerobic metabolism in response to copper exposure in laboratory conditions

Virginie Maes (1) , Stéphane Betoulle (1) , Ali Jaffal (1) , Odile Dedourge-Geffard (1) , Laurence Delahaut (1) , Alain Geffard (1) , Olivier Palluel (2) , Wilfried Sanchez (2) , Séverine Paris-Palacios (1) , Aurélie Vettier (1) , Elise David (1)

1 SEBIO - Stress Environnementaux et BIOsurveillance des milieux aquatiques
2 INERIS - Institut National de l'Environnement Industriel et des Risques

This study aims to determine the potential impairment of cell energy synthesis processes (glycolysis and respiratory chain pathways) by copper in juvenile roach at different regulation levels by using a multi-marker approach. Juvenile roach were exposed to 0, 10, 50, and 100 µg/L of copper for 7 days in laboratory conditions. The glycolysis pathway was assessed by measuring the relative expression levels of 4 genes encoding glycolysis enzymes. The respiratory chain was studied by assessing the electron transport system and cytochrome c oxidase gene expression. Muscle mitochondria ultrastructure was studied, and antioxidant responses were measured. Furthermore, the main energy reserves—carbohydrates, lipids, and proteins—were measured, and cellular energy was evaluated by measuring ATP, ADP, AMP and IMP concentrations. This study revealed a disturbance of the cell energy metabolism due to copper exposure, with a significant decrease in adenylate energy charge in roach exposed to 10 μg/L of copper after 1 day. Moreover, ATP concentrations significantly decreased in roach exposed to 10 μg/L of copper after 1 day. This significant decrease persisted in roach exposed to 50 µg/L of copper after 7 days. AMP concentrations increased in all contaminated fish after 1 day of exposure. In parallel, the relative expression of 3 genes encoding for glycolysis enzymes increased in all contaminated fish after 1 day of copper exposure. Focusing on the respiratory chain, cytochrome c oxidase gene expression also increased in all contaminated fish at the two time-points. The activity of the electron transport system was not disturbed by copper, except in roach exposed to 100 µg/L of copper after 1 day. Copper induced a metabolic stress. Juvenile roach seemed to respond to the ensuing high energy demand by increasing their anaerobic metabolism, but the energy produced by the anaerobic metabolism is unable to compensate for the stress induced by copper after 7 days. This multi-marker approach allows us to reach a greater understanding of the effects of copper on the physiological responses of juvenile roach.

Mots clés: ENERGY METABOLISM; ROACH; COPPER; GLYCOLYSIS; RESPIRATORY CHAIN

Domaines: Ecotoxicologie

Virginie Maes, Stéphane Betoulle, Ali Jaffal, Odile Dedourge-Geffard, Laurence Delahaut, et al.. Juvenile roach (Rutilus rutilus) increase their anaerobic metabolism in response to copper exposure in laboratory conditions. Ecotoxicology, 2016, 25 (5), pp.900-913. ⟨10.1007/s10646-016-1648-4⟩. ⟨ineris-01854128⟩