The three spined-stickleback's digestive enzymes as new biomarkers in environmental monitoring: effect of cadmium and temperature

Abstract : The three-spined stickleback, is a freshwater, brackish, and marine fish species with large distribution throughout the northern Hemisphere. This pollution-tolerant fish is present in most European streams and small rivers but also coastal and estuarian areas, which make it a suitable candidate for environmental monitoring and risk assessment of aquatic ecosystems. Several biomarkers have been developed for this animal model. This study aims at characterizing and developing a new category of biomarkers in relation with energy acquisition, in sticklebacks. Among these parameters, digestive capacities of many invertebrates and vertebrates aquatic organisms have demonstrated a good sensitivity to contaminants. Thus, in this context, the most important digestive enzymes were firstly characterized in this sentinel species, according to it diet, in optimal living conditions. Secondly male and female juvenile sticklebacks were exposed for 3 months in semi-static conditions to cadmium at 1µg.L-1 (chemical stress), and two temperature values 16°C and 21°C (physical stress). Water quality and cadmium concentration in the water were also monitored throughout the experiment. Parameters such as somatic indexes (height, weight, fulton’s condition index) and digestive enzymes (trypsin, intestinal alkaline phosphatase and amylase) were measured at 15, 60 and 90 days interval after cadmium exposure. The results have demonstrated a significant decrease in biometric parameters, from the 3rd month, especially among groups subjected to a temperature of 21 ° C. Furthermore, we observed an important alteration of the activity of the different enzymes over time and especially for trypsin and alkaline phosphatase in individuals exposed to cadmium at 21°C suggesting interactions between chemical and physical stresses on biological responses. For the first time, the digestive enzymes of the three-spined stickleback are reported as new environmental biomarkers.
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Submitted on : Monday, August 6, 2018 - 3:16:51 PM
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  • HAL Id : ineris-01854216, version 1

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Younes Hani, Cyril Turies, Elodie Kerambrun, Odile Dedourge-Geffard, Jean-Marc Porcher, et al.. The three spined-stickleback's digestive enzymes as new biomarkers in environmental monitoring: effect of cadmium and temperature. 26. SETAC Europe annual meeting, May 2016, Nantes, France. ⟨ineris-01854216⟩

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