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Cell-specific biotransformation of benzophenone-2 and bisphenol-s in zebrafish and human in vitro models used for toxicity and estrogenicity screening

Vincent Le Fol 1, 2 Selim Ait-Aissa 1 Nicolas J. Cabaton 3 Laurence Dolo 2 Marina Grimaldi 4 Patrick Balaguer 4 Elisabeth Perdu 2 Laurent Debrauwer 5 François Brion 1 Daniel Zalko 3
1 INERIS - Institut National de l'Environnement Industriel et des Risques
2 ToxAlim - ToxAlim
3 ToxAlim-MeX - Métabolisme et Xénobiotiques
ToxAlim - ToxAlim
4 IRCM - U1194 Inserm - UM - Institut de Recherche en Cancérologie de Montpellier
5 E20 Metatoul-AXIOM - Analyse de Xénobiotiques, Identification, Métabolisme
ToxAlim - ToxAlim, MetaToul-MetaboHUB
Abstract : Several human and fish bioassays have been designed to characterize the toxicity and the estrogenic activity of chemicals. However, their biotransformation capability (bioactivation/detoxification processes) is rarely reported, although this can influence the estrogenic potency of test compounds. The fate of two estrogenic chemicals, the UV;filter benzophenone-2 (BP2) and the bisphenol A substitute bisphenol S (BPS) was deciphered in eight human and zebrafish in vitro Cell models, encompassing hepatic and mammary Cellular contexts. BP2 and BPS were metabolized into a variety of glueo- and sulfo-conjugated metabolites. Similar patterns of BP2 and BPS biotransformation Were observed among zebrafish models (primary hepatocytes, ZFL and ZELH-zfER cell lines). Interestingly, metabolic patterns in zebrafigh models and in the human hepatic cell line HepaRG shared many similarities, while biotransformation rates in cell lines widely used for estrogenicity testing (MELN and T47D-KBLuc) were quantitatively low and qualitatively different. This study provides new data on the comparative metabolism of BP2 and BPS in human and fish cellular models that will help characterize their metabolic capabilities, and underlines the relevance of using in vitro ebrafish-based bioassays when screening for endocrine disrupting chemicals.
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Journal articles
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https://hal-ineris.archives-ouvertes.fr/ineris-01855040
Contributor : Gestionnaire Civs <>
Submitted on : Tuesday, August 7, 2018 - 1:40:30 PM
Last modification on : Friday, July 16, 2021 - 3:15:24 AM

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INERIS | INRA | VALDAURELLE | FNCLCC | UNIV-MONTPELLIER | BS | IRCM | UNIV-TLSE3 | INRAE | TOXALIM | TOXALIM-METATOUL-AXIOM | TOXALIM-MEX | INSA-TOULOUSE

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Vincent Le Fol, Selim Ait-Aissa, Nicolas J. Cabaton, Laurence Dolo, Marina Grimaldi, et al.. Cell-specific biotransformation of benzophenone-2 and bisphenol-s in zebrafish and human in vitro models used for toxicity and estrogenicity screening. Environmental Science and Technology, American Chemical Society, 2015, 49 (6), pp.3860-3868. ⟨10.1021/es505302c⟩. ⟨ineris-01855040⟩

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