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Journal articles
Peptide Anchor for Folate-Targeted Liposomal Delivery
Eugenia Nogueira
Irene C. Mangialavori
Ana Loureiro
Nuno G. Azoia
Marisa P. Saarria
Patricia Nogueira
Jaime Freitas
Johan Harmark
Ulyana Shimanovich
Alexandra Rollett
1
Ghislaine Lacroix
2
Goncalo J.L. Bernardes
Georg Guebitz
1
Hans Hebert
Alexandra Moreira
Alexandre M. Carmo
Juan Pablo Rossi
Andreia C. Gomes
Ana Preto
Artur Cavaco-Paulo
Ulyana Shimanovich
Author
Alexandra Rollett 1
Author
Ghislaine Lacroix 2
Author
Goncalo J.L. Bernardes
Author
1
BOKU - University of Natural Resources and Applied Life Sciences (Gregor Mendel Straße 33, 1180 ViennaAustria - Austria)
2
INERIS - Institut National de l'Environnement Industriel et des Risques (Verneuil-en-Halatte ; Nancy ; Aix en Provence - France)
Abstract : Specific folate receptors are abundantly overexpressed in chronically activated macrophages and in most cancer cells. Directed folate receptor targeting using liposomes is usually achieved using folate linked to a phospholipid or cholesterol anchor. This link is formed using a large spacer like polyethylene glycol. Here, we report an innovative strategy for targeted liposome delivery that uses a hydrophobic fragment of surfactant protein D linked to folate. Our proposed spacer is a small 4 amino acid residue linker. The peptide conjugate inserts deeply into the lipid bilayer without affecting liposomal integrity, with high stability and specificity. To compare the drug delivery potential of both liposomal targeting systems, we encapsulated the nuclear dye Hoechst 34580. The eventual increase in blue fluorescence would only be detectable upon liposome disruption, leading to specific binding of this dye to DNA. Our delivery system was proven to be more efficient (2-fold) in Caco-2 cells than classic systems where the folate moiety is linked to liposomes by polyethylene glycol.
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https://hal-ineris.archives-ouvertes.fr/ineris-01855132
Contributor : Gestionnaire Civs <>
Submitted on : Tuesday, August 7, 2018 - 2:24:45 PM
Last modification on : Saturday, June 15, 2019 - 8:06:09 PM
Contributor : Gestionnaire Civs <>
Submitted on : Tuesday, August 7, 2018 - 2:24:45 PM
Last modification on : Saturday, June 15, 2019 - 8:06:09 PM
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- HAL Id : ineris-01855132, version 1
- DOI : 10.1021/acs.biomac.5b00823
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Eugenia Nogueira, Irene C. Mangialavori, Ana Loureiro, Nuno G. Azoia, Marisa P. Saarria, et al.. Peptide Anchor for Folate-Targeted Liposomal Delivery. Biomacromolecules, American Chemical Society, 2015, 16 (9), pp.2904-2910. ⟨10.1021/acs.biomac.5b00823⟩. ⟨ineris-01855132⟩
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