Inter-laboratory trial of a standardized sediment contact test with the aquatic plant Myriophyllum aquaticum (ISO 16191) - Archive ouverte HAL Access content directly
Journal Articles Environmental Toxicology and Chemistry Year : 2014

Inter-laboratory trial of a standardized sediment contact test with the aquatic plant Myriophyllum aquaticum (ISO 16191)

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Ute Feiler
  • Function : Author
Monika Ratte
  • Function : Author
Gertie Arts
  • Function : Author
Christine Bazin
  • Function : Author
Frank Brauer
  • Function : Author
Laszlo Doren
  • Function : Author
Britta Eklund
  • Function : Author
Daniel Gilberg
  • Function : Author
Matthias Grote
  • Function : Author
Guido Gonsior
  • Function : Author
Christoph Hafner
  • Function : Author
Willi Kopf
  • Function : Author
Bernd Lemnitzer
  • Function : Author
Anja Liedtke
  • Function : Author
Uwe Matthias
  • Function : Author
Ewa Okos
  • Function : Author
Dirk Scheerbaum
  • Function : Author
Kathleen Stewart
  • Function : Author
Ivana Teodorovic
  • Function : Author
Andrea Wensel
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Hans-Juergen Pluta
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Abstract

A whole-sediment toxicity test with Myriophyllum aquaticum has been developed by the German Federal Institute of Hydrology and standardized within the International Organization for Standardization (ISO; ISO 16191). An international ring-test was performed to evaluate the precision of the test method. Four sediments (artificial, natural) were tested. Test duration was 10 d, and test endpoint was inhibition of growth rate (r) based on fresh weight data. Eighteen of 21 laboratories met the validity criterion of r >= 0.09 d(-1) in the control. Results from 4 tests that did not conform to test-performance criteria were excluded from statistical evaluation. The inter-laboratory variability of growth rates (20.6%-25.0%) and inhibition (26.6%-39.9%) was comparable with the variability of other standardized bioassays. The mean test-internal variability of the controls was low (7% [control], 9.7% [solvent control]), yielding a high discriminatory power of the given test design (median minimum detectable differences [MDD] 13% to 15%). To ensure these MDDs, an additional validity criterion of CV <= 15% of the growth rate in the controls was recommended. As a positive control, 90 mg 3,5-dichlorophenol/kg sediment dry mass was tested. The range of the expected growth inhibition was proposed to be 35 +/- 15%. The ring test results demonstrated the reliability of the ISO 16191 toxicity test and its suitability as a tool to assess the toxicity of sediment and dredged material.

Dates and versions

ineris-01855481 , version 1 (08-08-2018)

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Ute Feiler, Monika Ratte, Gertie Arts, Christine Bazin, Frank Brauer, et al.. Inter-laboratory trial of a standardized sediment contact test with the aquatic plant Myriophyllum aquaticum (ISO 16191). Environmental Toxicology and Chemistry, 2014, 33 (3), pp.662-670. ⟨10.1002/etc.2483⟩. ⟨ineris-01855481⟩
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