Identification of synthetic steroids in river water downstream from pharmaceutical manufacture discharges based on a bioanalytical approach and passive sampling

Nicolas Creusot; Selim Ait-Aissa; Nathalie Tapie; Patrick Pardon; François Brion; Wilfried Sanchez; Eric Thybaud; Jean-Marc Porcher; Hélène Budzinski

Identification of synthetic steroids in river water downstream from pharmaceutical manufacture discharges based on a bioanalytical approach and passive sampling

Nicolas Creusot ¹ Selim Ait-Aissa ¹ Nathalie Tapie ² Patrick Pardon ² François Brion ¹ Wilfried Sanchez ^{1, 3} Eric Thybaud ¹ Jean-Marc Porcher ^{1, 3} Hélène Budzinski ²

1 INERIS - Institut National de l'Environnement Industriel et des Risques

2 LPTC - Laboratoire de Physico -& Toxico Chimie des systèmes naturels

3 SEBIO - Stress Environnementaux et BIOsurveillance des milieux aquatiques

Abstract : A bioanalytical approach was used to identify chemical contaminants at river sites located downstream from a pharmaceutical factory, where reproductive alterations in wild fish have been previously observed. By using polar organic compound integrative samplers (POCIS) at upstream and downstream sites, biological activity profiles based on in vitro bioassays revealed the occurrence of xenobiotic and steroid-like activities, including very high glucocorticoid, antimineralocorticoid, progestogenic and pregnane X receptor (PXR)-like activities (mu g standard-EQ/g of sorbent range), and weak estrogenic activity (ng E2-EQ/g of sorbent range). Chemical analyses detected up to 60 out of 118 targeted steroid and pharmaceutical compounds in the extracts. In vitro profiling of occurring individual chemicals revealed the ability of several ones to act as agonist and/or antagonist of different steroids receptors. Mass balance calculation identified dexamethasone, spironolactone, and 6-alpha-methylprednisolone as major contributors to corticosteroid activities and levonorgestrel as the main contributor to progestogenic activities. Finally, RP-HPLC based fractionation of POCIS extracts and testing activity of fractions confirmed identified compounds and further revealed the presence of other unknown active chemicals. This study is one of the first to report environmental contamination by such chemicals; their possible contribution to in situ effects on fish at the same site is suggested.

Document type :

Journal articles

Domain :

Life Sciences [q-bio] / Toxicology / Ecotoxicology

Complete list of metadatas

https://hal-ineris.archives-ouvertes.fr/ineris-01855483
Contributor : Gestionnaire Civs <>
Submitted on : Wednesday, August 8, 2018 - 11:12:00 AM
Last modification on : Wednesday, March 27, 2019 - 10:58:08 AM

Identification of synthetic steroids in river water downstream from pharmaceutical manufacture discharges based on a bioanalytical approach and passive sampling

Identifiers

Collections

Citation

Export

Metrics

41

Contact

Identification of synthetic steroids in river water downstream from pharmaceutical manufacture discharges based on a bioanalytical approach and passive sampling

Identifiers

Collections

Citation

Export

Share

Metrics

41

Contact