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Article Dans Une Revue NanoImpact Année : 2018

Towards reproducible measurement of nanoparticle size using dynamic light scattering: Important controls and considerations

Résumé

It is prerequisite to characterize nanoparticles in dispersions in particular with respect to their size and size distribution before they can be used in toxicological testing. This requires robust methods with high reproducibility. The aim of this study was to apply interlaboratory comparisons using Dynamic Light Scattering (DLS), in order to evaluate the reproducibility of nanoparticle size determinations. DLS is well established in most nanotoxicological laboratories. Due to the fact that this technique appears easy to use it has become a standard technique nowadays. Still, reproducibility and in particular differences between different commercial instruments have not been addressed systematically before. Our aim was to develop a robust standard operating procedure (SOP). Therefore, at the beginning, experiments were performed with dispersions of rather monodisperse and spherical particles in water. Significant differences were observed between the results obtained with different commercial instruments for DLS, especially when fitting the data using mathematical inversion methods. As a consequence of the different settings applied when fitting the data, significant errors could be made especially when working with dilute dispersions of very small particles. Here we have identified options how to overcome possible issues. However, It should be noted, that in practice, nanoparticles may have significant polydispersities and/or be non-spherical. The potential of the DLS technique in this situation should be noted that in nanoparticles may have significant polydispersities and/or be non-spherical. The potential of the DLS technique in this situation is evaluated. A combination of two different techniques (DLS and electron microscopy for instance) is recommended to fully interpret the results.
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Dates et versions

hal-04037015 , version 1 (20-03-2023)

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Dominique Langevin, Eric Raspaud, Sandrine Mariot, A. Knyazev, A. Stocco, et al.. Towards reproducible measurement of nanoparticle size using dynamic light scattering: Important controls and considerations. NanoImpact, 2018, 10, pp.161-167. ⟨10.1016/j.impact.2018.04.002⟩. ⟨hal-04037015⟩
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