, (5Á9) Dissolved oxygen (mg/l) 12.3 (10.9Á14.2) 11.6 (9.2Á12.6) 11.9 (8.6Á18.4) Conductivity (mS/cm) 70 (62Á97) 71.9 (46Á83) 146.4 (96Á182) Cl

, 95) Total iron (mg/l) 0.1 (0, 0Á120) 39.4 (3Á103) 42.2 (1Á147) Phenolic compounds

. Cadmium, 03 (ndÁ0.09) 0.05 (ndÁ0.34) NA a Copper (mg/l) 1.11 (ndÁ2.65) 1.19 (ndÁ6.84) NA a a Water sampled in Ferme Pond in 2005 presented metal concentration B 2 mg/L for copper and B 0.1 mg/L for cadmium, but such results were not recorded on a monthly basis

, Jaffal et al. 1043 (14Á2693) b,c Numbers of melanomacrophage centres (/mm 2 ) 1.6 (0.6Á3.5) a,b 3, Metal contamination and hepatic toxicological responses in brown trout A2Á4.9) b 1.9 (0.4Á3) a

, 8 (1Á4.3) a 35Á5) a,b 31Á5) a,b Vacuolization scores 1.7 (0Á4.3) a,b 0.5 (0Á3.1) a 2) a,b Fibrosis scores 2, Nuclear alteration scores 4.2 (3.6Á5) b 33Á5) b 2.7 (1Á5) b 1.5 (0Á4.5) a

, Immune cell infiltration scores 1.8 (0.1Á2.6) a 2.6 (1.5Á3.8) a 2.3 (1Á3.1) a 1.7 (0Á3.3) a 1.6 (0.1Á3) a 2.2 (1Á3.8) a Global scores 2.2 (1.2Á3.2) a 2.3 (1.7Á3.1) a 2.4 (1.5Á3.3) a 2.3 (1.5Á3.2) a 1.9 (1Á2.7) a 2.3 (1.4Á3.5) a

, References

O. Abollino, M. Aceto, S. Buoso, M. Gasparon, W. J. Green et al., Distribution of major, minor and trace elements in lake environments of Antarctica, Antarctic Science, vol.16, issue.3, pp.277-291, 2004.
DOI : 10.1017/S0954102004002111

S. M. Adams, K. D. Ham, M. S. Greeley, R. F. Lehew, D. E. Hinton et al., Downstream gradients in bioindicator responses: point source contaminant effects on fish health, Canadian Journal of Fisheries and Aquatic Sciences, vol.37, issue.10, pp.2177-2187, 1996.
DOI : 10.1139/f80-017

C. Agius, Preliminary Studies on the Ontogeny of the Melano-Macrophages of Teleost Haemopoietic Tissues and Age-Related Changes, Developmental & Comparative Immunology, vol.5, issue.4, pp.597-606, 1981.
DOI : 10.1016/S0145-305X(81)80034-1

S. Ait-aissa, O. Ausseil, O. Palluel, E. Vindimian, J. Garnier-laplace et al., ) after single and combined exposure to low doses of cadmium, zinc, PCB77 and 17??-oestradiol, Biomarkers, vol.19, issue.6, pp.77-94, 2003.
DOI : 10.1016/0166-445X(91)90033-6

P. Amundsen, F. J. Staldvik, A. A. Lukin, N. A. Kashulin, O. A. Popova et al., Heavy metal contamination in freshwater fish from the border region between Norway and Russia, Science of The Total Environment, vol.201, issue.3, pp.211-224, 1997.
DOI : 10.1016/S0048-9697(97)84058-2

S. Babo and P. Vasseur, In vitro effects of Thiram on liver antioxidant enzyme activities of rainbow trout (Oncorhynchus mykiss) Aquatic Toxicology 22, pp.61-68, 1992.

R. Bargagli, Environmental contamination in Antarctic ecosystems, Science of The Total Environment, vol.400, issue.1-3, pp.212-226, 2008.
DOI : 10.1016/j.scitotenv.2008.06.062

E. Baysoy, G. Atli, C. Gü-rler, Z. Dogan, A. Eroglu et al., The effects of increased freshwater salinity in the biodisponibility of metals (Cr, Pb) and effects on antioxidant systems of Oreochromis niloticus, Ecotoxicology and Environmental Safety, vol.84, pp.249-253, 2012.
DOI : 10.1016/j.ecoenv.2012.07.017

D. Bernet, H. Schmidt-posthaus, T. Wahli, and P. Burkhardt-holm, Evaluation of Two Monitoring Approaches to Assess Effects of Waste Water Disposal on Histological Alterations in Fish, Hydrobiologia, vol.524, issue.1, pp.53-66, 2004.
DOI : 10.1023/B:HYDR.0000036196.84682.27

S. Betoulle, C. Duchiron, and P. Deschaux, Lindane increases in vitro respiratory burst activity and intracellular calcium levels in rainbow trout (Oncorhynchus mykiss) head kidney phagocytes, Aquatic Toxicology, vol.48, issue.2-3, pp.211-221, 2000.
DOI : 10.1016/S0166-445X(99)00041-7

S. Biagianti-risbourg, Contribution a` l'e´tude du foie de juve´niles de muges (Te´le´oste´ens, Mugilides), contamine´s expe´rimentalement par l'atrazine (s-triazine herbicide): approche ultrastructurale et me´tabolique: inte´re?t en e´cotoxicologie. (Study of juveniles mullet [Teleostei, Mugilidae] liver contaminated by atrazine [s-triazine herbicide]: ultrastructural and metabolic approach: ecotoxicological interest, 1990.

J. D. Borucinska, K. Kotran, M. Shackett, and T. Barker, (Bonnaterre), Isurus oxyrhinchus Rafinesque, and the thresher, pp.883-891, 2009.
DOI : 10.1016/S0145-305X(81)80034-1

M. M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Analytical Biochemistry, vol.72, issue.1-2, pp.248-254, 1976.
DOI : 10.1016/0003-2697(76)90527-3

T. Braunbeck, Cytological alterations in fish hepatocytes following in vivo and in vitro sublethal exposure to xenobiotics ??? structural biomarkers of environmental contamination, 1998.
DOI : 10.1007/978-3-0348-8853-0_3

. Braunbeck, Fish ecotoxicology, Pp, pp.61-140
DOI : 10.1007/978-3-0348-8853-0

K. Broeg, The activity of macrophage aggregates in the liver of flounder (Platichthys flesus) and wrasse (Symphodus melops) is associated with tissue damage, Marine Environmental Research, vol.69, pp.14-16, 2010.
DOI : 10.1016/j.marenvres.2009.11.010

J. Bruslé and G. G. Anadon, The structure and function of fish liver, Fish morphology. Pp. 77Á93. Boca Raton, 1996.

J. Bruslé and J. P. Quignard, Biologie des poissons d'eau douce europe´ens, Biology of European freshwater fish.) Paris: Lavoisier, 2001.

P. Bryè-re and M. Charrier, Variations du re´gime alimentaire en fonction de l'habitat de la truite commune (Salmo trutta L.) introduite dans l'Archipel de Kerguelen, Diet variations depending on the habitat of brown trout [Salmo trutta L.] introduced into the Kerguelen archipelago.) Master's thesis, 2009.

P. Bustamante, P. Bocher, Y. Chérel, P. Miramand, and F. Caurant, Distribution of trace elements in the tissues of benthic and pelagic fish from the Kerguelen Islands, Science of The Total Environment, vol.313, issue.1-3, pp.25-39, 2003.
DOI : 10.1016/S0048-9697(03)00265-1
URL : https://hal.archives-ouvertes.fr/hal-00186633

P. Bustamante, Y. Cherel, F. Caurant, and P. Miramand, Cadmium, copper and zinc in octopuses from Kerguelen Islands, Southern Indian Ocean, Polar Biology, vol.19, issue.4, pp.264-271, 1998.
DOI : 10.1007/s003000050244
URL : https://hal.archives-ouvertes.fr/hal-00186628

J. Carrola, A. Fontainhas-fernandes, P. Matos, and E. Rocha, Liver Histopathology in Brown Trout (Salmo trutta f. fario) from the Tinhela River, Subjected to Mine Drainage from the Abandoned Jales Mine (Portugal), Bulletin of Environmental Contamination and Toxicology, vol.6, issue.76, pp.35-41, 2009.
DOI : 10.1080/02705060.2000.9663741

J. Cazenave, C. Bacchetta, A. Rossi, A. Ale, M. Campana et al., Deleterious effects of wastewater on the health status of fish: A field caging study, Ecological Indicators, vol.38, pp.104-112, 2014.
DOI : 10.1016/j.ecolind.2013.10.029

P. Davaine, E. Beall, O. Guerri, and J. M. Caraguel, Salmonid introductions into virgin ecosystems (Kerguelen islands, Subantarctic): stakes, results, prospects. Bulletin Français de la Pe?che et de la Pisciculture 344, pp.93-110, 1997.

, EC of the European Parliament on the quality of fresh waters needing protection or improvement in order to support fish life. Accessed on the internet at http://eur-lex. europa, pp.32006-32050, 2006.

M. S. Evans, D. Muir, W. L. Lockhart, G. Stern, M. Ryan et al., Persistent organic pollutants and metals in the freshwater biota of the Canadian Subarctic and Arctic: An overview, Science of The Total Environment, vol.351, issue.352, pp.94-147, 2005.
DOI : 10.1016/j.scitotenv.2005.01.052

A. Farkas, J. Salá-nki, and A. Specziá-r, Age- and size-specific patterns of heavy metals in the organs of freshwater fish Abramis brama L. populating a low-contaminated site, Water Research, vol.37, issue.5, pp.959-964, 2003.
DOI : 10.1016/S0043-1354(02)00447-5

J. A. Ferná-ndez, M. D. Vázquez, J. Ló-pez, and A. Carballeira, Modelling the extra and intracellular uptake and discharge of heavy metals in Fontinalis antipyretica transplanted along a heavy metal and pH contamination gradient, Environmental Pollution, vol.139, issue.1, pp.21-31, 2006.
DOI : 10.1016/j.envpol.2005.04.036

C. Gay, Ecologie du zooplancton d'eau douce des Iles Kerguelen. Caracté ristiques du milieu et inventaire des entomostracé s. (Ecology of freshwater zooplankton of the Kerguelen Islands. Environmental characteristics and entomostraca inventory, pp.47-90, 1981.

B. K. Greenfield, S. J. Teh, J. R. Ross, J. Hunt, G. Zhang et al., Contaminant Concentrations and Histopathological Effects in Sacramento Splittail (Pogonichthys macrolepidotus), Archives of Environmental Contamination and Toxicology, vol.13, issue.2, pp.270-281, 2008.
DOI : 10.1007/s00244-007-9112-3

S. ¸. Gü-l, . Belge-kurutas¸ekurutas¸kurutas¸e, E. Y?ld?z, A. Doran, and F. , Pollution correlated modifications of liver antioxidant systems and histopathology of fish (Cyprinidae) living in Seyhan Dam Lake, Turkey, Environment International, vol.30, issue.5, pp.605-609, 2004.
DOI : 10.1016/S0160-4120(03)00059-X

D. E. Hinton and D. J. Lauré-n, Liver structural alterations accompanying chronic toxicity in fishes: potential biomarkers of exposure, Biomarkers of environmental contamination. Pp. 15Á57. Boca, 1990.

A. Jaffal, N. Givaudan, S. Betoulle, A. Terreau, S. Paris-palacios et al., Polychlorinated biphenyls in freshwater salmonids from the Kerguelen Islands in the Southern Ocean, Environmental Pollution, vol.159, issue.5, 2011.
DOI : 10.1016/j.envpol.2011.01.003
URL : https://hal.archives-ouvertes.fr/hal-00653911

A. Jaffal, S. Paris-palacios, S. Jolly, A. Thailly, L. Delahaut et al., Cadmium and copper contents in a freshwater fish species (brook trout, Salvelinus fontinalis) from the subantarctic Kerguelen Islands, Polar Biology, vol.53, issue.352, pp.397-409, 2011.
DOI : 10.1016/S0045-6535(03)00551-4
URL : https://hal.archives-ouvertes.fr/ineris-00963280

J. C. Johnson, S. R. Nettikadan, S. G. Vengasandra, S. Lovan, J. Muys et al., Characterization of testudine melanomacrophage linear, membrane extension processes*cablepodia*by phase and atomic force microscopy, Vitro Cellular and Developmental Biology* Animal 41, pp.225-231, 2005.
DOI : 10.1290/0504027
URL : http://static.springer.com/sgw/documents/374098/application/pdf/How+to+use+my+Springer+Token+for+Societies_2.pdf

J. Kojadinovic, M. Potier, L. Corre, M. Cosson, R. P. Bustamante et al., Bioaccumulation of trace elements in pelagic fish from the Western Indian Ocean, Environmental Pollution, vol.146, issue.2, pp.548-566, 2007.
DOI : 10.1016/j.envpol.2006.07.015
URL : https://hal.archives-ouvertes.fr/hal-00321778

S. Kolayli and E. Keha, A comparative study of antioxidant enzyme activities in freshwater and seawater-adapted rainbow trout, Journal of Biochemical and Molecular Toxicology, vol.36, issue.6, pp.334-371, 1999.
DOI : 10.3109/00498258609038976

L. Lagadic, T. Caquet, and J. C. Amiard, Biomarqueurs en e ´ cotoxicologie: principes et définitions. (Biomarkers in ecotoxicology: principles and definitions, Biomarqueurs en e´cotoxicologie. Aspects fondamentaux . (Biomarkers in ecotoxicology. Fundamental aspects.) Pp. 1Á9, 1997.

S. Lamas, J. A. Fernández, J. R. Aboal, and A. Carballeira, Testing the use of juvenile Salmo trutta L. as biomonitors of heavy metal pollution in freshwater, Chemosphere, vol.67, issue.2, pp.221-228, 2007.
DOI : 10.1016/j.chemosphere.2006.10.040

A. , Metal contamination and hepatic toxicological responses in brown trout Citation: Polar Research, 2015.

M. Manera, R. Serra, G. Isani, and E. Carpené, Macrophage aggregates in gilthead sea bream fed copper, iron and zinc enriched diets, Journal of Fish Biology, vol.31, issue.2, pp.457-465, 2000.
DOI : 10.1016/0145-305X(91)90007-L

M. J. Marchand, J. C. Van-dyk, G. M. Pieterse, I. E. Barnhoorn, and M. S. Bornman, from polluted aquatic systems in South Africa, Environmental Toxicology, vol.161, issue.2, pp.133-147, 2009.
DOI : 10.1139/f83-060

N. Mayon, A. Bertand, D. Leroy, C. Malbrouck, S. N. Mandiki et al., Multiscale approach of fish responses to different types of environmental contaminations: A case study, Science of The Total Environment, vol.367, issue.2-3, pp.715-731, 2006.
DOI : 10.1016/j.scitotenv.2006.03.005

J. N. Moore, S. N. Luoma, and D. Peters, Downstream Effects of Mine Effluent on an Intermontane Riparian System, Canadian Journal of Fisheries and Aquatic Sciences, vol.37, issue.2, pp.222-232, 1991.
DOI : 10.1139/f79-042

F. Morat, S. Betoulle, M. Robert, A. F. Thailly, S. Biagianti-risbourg et al., What can otolith examination tell us about the level of perturbations of salmonid fish from the Kerguelen Islands? Ecology of Freshwater Fish 17, pp.617-627, 2008.

F. Paoletti, D. Aldinucci, A. Mocali, and A. Caparrini, A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts, Analytical Biochemistry, vol.154, issue.2, pp.536-541, 1986.
DOI : 10.1016/0003-2697(86)90026-6

S. Paris-palacios and S. Biagianti-risbourg, Hepatocyte nuclear structure and subcellular distribution of copper in zebrafish Brachydanio rerio and roach Rutilus rutilus (Teleostei, Cyprinidae) exposed to copper sulphate, Aquatic Toxicology, vol.77, issue.3, pp.306-313, 2006.
DOI : 10.1016/j.aquatox.2006.01.001

S. Paris-palacios, S. Biagianti-risbourg, and G. Vernet, Hepatic disorders induced by a sublethal concentration of procymidone (fongicide dicarboximide) in Brachydanio rerio (teleostei, cyprinidae): histo-cytological approach, Bulletin Français, pp.547-562, 1998.

S. Paris-palacios, S. Biagianti-risbourg, and G. Vernet, Biochemical and (ultra)structural hepatic perturbations of Brachydanio rerio (Teleostei, Cyprinidae) exposed to two sublethal concentrations of copper sulfate, Aquatic Toxicology, vol.50, issue.1-2, pp.109-124, 2000.
DOI : 10.1016/S0166-445X(99)00090-9

J. Peute, R. Huiskamp, and P. Van-oordt, Quantitative analysis of estradiol-17??-induced changes in the ultrastructure of the liver of the male zebrafish, Brachydanio rerio, Cell and Tissue Research, vol.242, issue.2, pp.377-382, 1985.
DOI : 10.1007/BF00214551

W. Sanchez, S. Ait-aissa, O. Palluel, J. M. Ditche, and J. M. Porcher, Preliminary investigation of multi-biomarker responses in three-spined stickleback (Gasterosteus aculeatus L.) sampled in contaminated streams, Ecotoxicology, vol.51, issue.366, pp.279-287, 2007.
DOI : 10.1029/TR038i006p00913
URL : https://hal.archives-ouvertes.fr/ineris-00961909

W. Sanchez, O. Palluel, L. Lagadic, S. Ait-aissa, and J. M. Porcher, Biochemical effects of nonylphenol polyethoxylate adjuvant, Diquat herbicide and their mixture on the threespined stickleback (Gasterosteus aculeatus L.). Marine Environmental Research 62, pp.29-33, 2006.

W. Sanchez, O. Palluel, L. Meunier, M. Coquery, J. Porcher et al., Copper-induced oxidative stress in three-spined stickleback: relationship with hepatic metal levels, Environmental Toxicology and Pharmacology, vol.19, issue.1, pp.177-183, 2005.
DOI : 10.1016/j.etap.2004.07.003
URL : https://hal.archives-ouvertes.fr/ineris-00961894

H. Schmidt, D. Bernet, T. Wahli, W. Meier, and P. Burkhardt-holm, Active biomonitoring with brown trout and rainbow trout in diluted sewage plant effluents, Journal of Fish Biology, vol.156, issue.3, pp.585-596, 1999.
DOI : 10.1007/BF00694263

H. Schmidt-posthaus, D. Bernet, T. Wahli, and P. Burkhardt-holm, Morphological organ alterations and infectious diseases in brown trout Salmo trutta and rainbow trout Oncorhynchus mykiss exposed to polluted river water, Diseases of Aquatic Organisms, vol.44, pp.161-170, 2001.
DOI : 10.3354/dao044161
URL : http://www.int-res.com/articles/dao/44/d044p161.pdf

J. Schwaiger, Histopathological alterations and parasite infection in fish: indicators of multiple stress factors, Journal of Aquatic Ecosystem Stress and Recovery, vol.8, issue.3/4, pp.231-240, 2001.
DOI : 10.1023/A:1012954608541

J. Schwaiger, R. Wanke, A. S. Pawert, M. Honnen, W. Triebskorn et al., The use of histopathological indicators to evaluate contaminant-related stress in fish, Journal of Aquatic Ecosystem Stress and Recovery, vol.6, issue.1, pp.75-85, 1997.
DOI : 10.1023/A:1008212000208

A. R. Schwindt, N. Truelove, C. B. Schreck, J. W. Fournie, D. H. Landers et al., Quantitative evaluation of macrophage aggregates in brook trout Salvelinus fontinalis and rainbow trout Oncorhynchus mykiss, Diseases of Aquatic Organisms, vol.68, pp.101-113, 2006.
DOI : 10.3354/dao068101
URL : http://www.int-res.com/articles/dao2005/68/d068p101.pdf

C. Vandeputte, I. Guizon, I. Genestie-denis, B. Vannier, and G. Lorenzon, A microtiter plate assay for total glutathione and glutathione disulfide contents in cultured/isolated cells: performance study of a new miniaturized protocol, Cell Biology and Toxicology, vol.69, issue.5-6, pp.415-421, 1994.
DOI : 10.1007/BF00755791

R. Van-der-oost, J. Beyer, and N. P. Vermeulen, Fish bioaccumulation and biomarkers in environmental risk assessment: a review, Environmental Toxicology and Pharmacology, vol.13, issue.2, pp.57-149, 2003.
DOI : 10.1016/S1382-6689(02)00126-6

W. Filho, D. Torres, M. A. Tribess, T. B. Pedrosa, R. C. Soares et al., Influence of season and pollution on the antioxidant defenses of the cichlid fish acar?? (Geophagus brasiliensis), Brazilian Journal of Medical and Biological Research, vol.120, issue.6, pp.719-726, 2001.
DOI : 10.1016/S1095-6433(98)10008-9