Écotoxicologie : effets des perturbateurs endocriniens sur la reproduction chez le poisson

Abstract : In recent years, it has been shown that man-made chemicals and natural substances are able to disrupt the normal physiology and endocrinology of organisms. The presence of these compounds, termed endocrine-disrupting chemicals (EDCs), has been a major concern for more than 10 years. Given the risk posed by EDCs on the reproductive function of organisms, the ecotoxicological risk assessment unit of INERIS initiated at the end of the 1990s research programmes aimed at developing in vitro and in vivo biological tools to detect and to characterise the endocrine disrupting potency of chemicals in fish with a view to assessing the hazards and the risks of EDCs. Our work initially focused on an important class of EDCs, i.e. those acting as estrogens (xeno-estrogens). However, EDCs can affect the endocrine system through various pathways and action mechanisms. Therefore, with the aim of broadening our knowledge on the effects of EDCs on fish development and reproduction, more recently we have conducted research that aims to investigate the effect of several classes of chemical compounds, on P450 aromatase, an essential sex-related enzyme involved in the final rate-limiting conversion of androgens into estrogens. By conducting in vitro and in vivo experiments in our model fish species, the zebrafish, we demonstrated that a wide range of compounds such as xeno-estrogens, dioxins or pesticides were able to perturb aromatase expression and/or activities by acting at the transcriptional level or by modulating aromatase activities. While the biological significance of these molecular and biochemical effects on fish development and reproduction need to be determined, aromatase could be integrated in new or existing in vitro/ in vivo fish assays to assess potential aquatic organism endocrine activity in a testing strategy that is required particularly within REACH.
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Nathalie Hinfray, Selim Ait-Aissa, Jean-Marc Porcher, François Brion. Écotoxicologie : effets des perturbateurs endocriniens sur la reproduction chez le poisson. Rapport Scientifique INERIS, 2007, 2006-2007, pp.34-36. ⟨ineris-01869083⟩

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