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Recherche et identification de polluants chimiques perturbateurs endocriniens dans les milieux aquatiques : approche bioanalytique

Abstract : Bio-analytical approaches that combine in vitro biological tools and chemical analyses have been developed and used to identifying endocrine disrupting chemicals (EDCs) and dioxin-like compounds in environmental matrices such as effluents, surface waters or river sediments. By using a panel of in vitro bioassays based on cultured reporter cell lines, our study revealed the presence of multiple endocrine active chemicals (i.e. chemicals that interfere with estrogen, androgenic and pregnane X receptors) and dioxin-like compounds in sediments from various French river sites. Chemicals analyses of several EDCs including steroid hormones, mycotoxins, UV-screens, alkylphenols, parabens, pesticides and PAHs confirmed multiple contamination at the most active sites as determined by the bioassays. On the basis of toxic-equivalent concentrations (TEQs), an overall good correlation was noted between biological and chemical approaches. For instance, PAHs were found as major contributors to the dioxin-like activities while the natural steroid estrogens, estradiol and estrone, explained most of the estrogenic activities. However, at the most impacted sites, targeted chemical analyses were only partly explicative of the biological activities measured in the samples suggesting that non analyzed pollutants were also present in the samples. Indeed, fractionation of the samples (RP-HPLC) and testing of the fractions with the bioassays revealed the presence of other unknown estrogenic chemicals that remain to be identified.
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  • HAL Id : ineris-01869238, version 1



Selim Ait-Aissa, Nicolas Creusot, Saïd Kinani, Jean-Marc Porcher. Recherche et identification de polluants chimiques perturbateurs endocriniens dans les milieux aquatiques : approche bioanalytique. Rapport Scientifique INERIS, 2009, 2008-2009, pp.18-20. ⟨ineris-01869238⟩



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