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Outils de criblage in vitro et in vivo chez le poisson pour l’évaluation des effets endocriniens de polluants aquatiques

Abstract : Current concern on the effects of endocrine disrupting chemicals (EDCs) to fish and human health has stimulated the development and implementation of screening and testing procedures, notably within the perspective of the new EU regulatory framework for chemicals (REACH). Given the complexity of modes of action and the effects of EDCs, various biological targets are to be evaluated at molecular, cellular, organism and population levels. Here, we set up a panel of new in vitro and in vivo mechanism-based tools to study chemical interaction with estrogen-regulated pathways in fish. By using stable reporter cell lines derived from fish and human cell lines, it is shown that estrogenic responses vary between models, likely due to cell context and/or cross-species differences. In addition, such in vitro screening of various environmental molecules allowed identifying those that exert higher estrogenic potency in fish cellular context, such as UV screens. To further assess such specific effects in vivo, we report the use of hormone-regulated gene expression and a transgenic line expressing green fluorescent protein under the control of brain aromatase promoter (cyp19a1b) that were developed in a model fish species, the zebrafish. The developed assays offer rapid, specific and sensitive in vitro and in vivo screening tools that could serve in EDCs testing strategies for aquatic vertebrates.
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Submitted on : Thursday, September 6, 2018 - 1:12:37 PM
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  • HAL Id : ineris-01869277, version 1



Selim Ait-Aissa, François Brion. Outils de criblage in vitro et in vivo chez le poisson pour l’évaluation des effets endocriniens de polluants aquatiques. Rapport Scientifique INERIS, 2010, 2009-2010, pp.54-56. ⟨ineris-01869277⟩



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