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Développement de méthodes alternatives en expérimentation animale : caractérisation de la toxicité et du métabolisme in vitro de xénobiotiques au sein de biopuces hépatiques et rénales

Abstract : Besides traditional in vitro systems such as plates or Petri dishes, microfluidic biochips have recently gained a remarkable interest. These in vitro systems aim at providing cellular environments close to in vivo conditions to reproduce reliably in vivo mechanisms or metabolic processes. In the framework of the SYSBIOX and μHepaReTox projects, we proposed to evaluate the toxicity and metabolism of xenobiotics in microfluidic biochips in order to assess the relevance of such systems for applications in toxicology and pharmacology. One of the major results was the development of the metabolomicson- chip approach. By combining the latter with the modeling of the intracellular metabolic network, we demonstrated that a microfluidic environment improves cell oxygenation. Our results indicated that the metabolic signature is unique to each biochip (liver, kidney or co-culture) demonstrating the presence of a specific endogenous metabolism directly related to the physiological function of the organ. In the presence of xenobiotics, a unique metabolic response, characteristic of each xenobiotic, is obtained in the hepatic biochip.
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Céline Brochot. Développement de méthodes alternatives en expérimentation animale : caractérisation de la toxicité et du métabolisme in vitro de xénobiotiques au sein de biopuces hépatiques et rénales. Rapport Scientifique INERIS, 2013, 2012-2013, pp.20-22. ⟨ineris-01869452⟩

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