Le modèle embryonnaire de poisson zèbre transgénique cyp19a1b-GFP

Abstract : Agonist ligands of estrogen receptors have been extensively studied in recent years because of their effects on reproduction and development of aquatic organisms such as fish. While there is a need to develop fish-specific mechanism-based bioassays to screen estrogenic potency of chemicals alone, it is also important to consider that organisms are often exposed to mixtures of endocrine disruptors (EDs) that are able to produce significant effects, even when each chemical is present at low doses that individually do not induce observable effects. We recently developed an in vivo mechanism-based assay called EASZY that uses transgenic zebrafish cyp19a1b-GFP embryos expressing GFP under the control of the cyp19a1b promoter. EASZY is a rapid and cost-effective assay allowing to screen estrogenic activity of chemicals and was further used to explore combined effect of ED. The tested mixtures of estrogenic compounds acted predominantly in an additive manner on the expression of GFP demonstrating that combined effects of estrogenic compounds can be predicted by using dose addition, although in some cases deviations were observed. The effects of combinations of EDs from different categories were also explored by testing a binary mixture of estradiol and testosterone. Overall, we show that the use of cyp19a1b-GFP zebrafish embryo assay combined with predictive modelling is relevant to further explore the effect of complex mixture of EDs.
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Cléo Tebby, François Brion, Nathalie Hinfray. Le modèle embryonnaire de poisson zèbre transgénique cyp19a1b-GFP. Rapport Scientifique INERIS, 2014, 2013-2014, pp.32-33. ⟨ineris-01869498⟩

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