Les tests in vitro à l’interface air-liquide permettent-ils de mieux prédire la toxicité pulmonaire des nanoparticules ?

Abstract : Nanoparticles (NPs) are potentially toxic for humans, especially after inhalation. The animal model is a relevant tool to predict potential adverse effects in human, because of a similar level of complexity exiting between the two models. Nevertheless, considering the high number of NPs used and their physicochemical diversity, it seems difficult to rely only on animal experimentation. In this context, it is necessary to find reliable models which can be used as alternative to the in vivo. The aim of this study was to assess the ability of several in vitro methods, including more realistic methods allowing exposure of lung cells at the air-liquid interface to aerosols of NPs, to predict the pulmonary adverse effects observed in vivo. For this purpose, in vitro and in vivo experiments were performed. It was observed that using more realistic in vitro methods can predict more closely the biological responses observed in vivo after acute exposure to NPs. This shows the relevance of developing and using more realistic in vitro methods and suggests perspectives regarding the implementation of standardized methodologies which could be used for in vitro based regulation.
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Thomas Loret, Ghislaine Lacroix. Les tests in vitro à l’interface air-liquide permettent-ils de mieux prédire la toxicité pulmonaire des nanoparticules ?. Rapport Scientifique INERIS, 2016, 2015-2016, pp.13-14. ⟨ineris-01869614⟩

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