Optimization of an active biomonitoring approach using three-spined sticklebacks

Audrey Catteau (1, 2) , Antoine Le Guernic (2) , Adrien Marchand (1) , Younes Hani (2) , Cyril Turies (1) , Olivier Palluel (1) , Anne Bado-Nilles (1) , Jean-Marc Porcher (1, 2)

1 INERIS - Institut National de l'Environnement Industriel et des Risques
2 SEBIO - Stress Environnementaux et BIOsurveillance des milieux aquatiques

Active biomonitoring involves exposure of calibrate and well-known organisms to environmental pollution and is increasingly used in ecological studies. Despite of the advantages of caging (standardization of some biotic and abiotic parameters), caged organisms are subject to some constraints which can influence the organisms’ responses to pollution and therefore can be considered as confounding factors. In the way to make biomarkers efficient tools in active biomonitoring, it is necessary to precisely characterize the possible effects of constraints linked to caging on organism physiology. For this purpose, several studies were conducted on threeBspined sticklebacks (Gasterosteus aculeatus) to specify the impacts of i) fish-density inside cages, ii) short-transport after caging, iii) movement limitation and iv) food access restriction induced by caging. The optimization of caging conditions has been validated by assessment of several biomarkers including innate immune responses, antioxidant systems, metabolic detoxification, synaptic transmission and energy metabolism. Results have shown that a high density of fish (> 2.31 kg/m3) and a short transport after caging have induced an oxidative stress manifested by an overproduction of reactive oxygen species and a stimulation of the antioxidant system. The confinement stress had small impact on sticklebacks’ biomarkers. However, this stress seemed to accentuate the negative effects of food restriction on these biomarkers, particularly during their breeding period. Out of breeding time, most of investigate biomarkers were not impacted by food access restriction. These works have allowed to specify the conditions of application and interpretation of biomarkers during active monitoring using stickleback to ensure a good diagnosis of the quality of water bodies with this species.

Domaines: Ecotoxicologie

Audrey Catteau, Antoine Le Guernic, Adrien Marchand, Younes Hani, Cyril Turies, et al.. Optimization of an active biomonitoring approach using three-spined sticklebacks. 9 International Conference on Stickleback Behaviour and Evolution (STICKLEBACK 2018), Jul 2018, Kyoto, Japan. ⟨ineris-03225036⟩