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Integrated Biological Approach to Characterize and Discriminate Contaminated Wasteland in the Context of Its Requalification to an Ecological Use

Abstract : Urban and industrial activities led to the discharge of a wide range of hazardous chemicals in soils. Among abandoned wasteland, those whose soil functions were not altered beyond repair may ends up hosting transitional ecosystems with ecological values. However, there is a lack of screening methodologies to characterize and categorize among these sites. The main concern is to differentiate the soils/sites which are potential candidates for immediate upgrading to an “ecological usage” (e.g. biodiversity reserve) from those that require a comprehensive ecological risk assessment (which is sometimes difficult to applied for economical and/or technical reasons). In this context, the project TIPOMO (transfer and index of concern: tools for valuing medium-contaminated wasteland) aims at building an “index of concern” that allow the categorization of polluted soils hosting transitional ecosystems. This index will be calculated by considering the levels of soil contamination and its ability to trap polluting substances (characterization of bioavailability fraction). This method will be tested and validated in real case study. To do so, an integrated approach based on pollution characterization, and biological endpoints (both ex situ and in situ) were applied on 16 soil composites collecting on 8 sites mainly contaminated with HAP and/or hydrocarbons and/or metals. The biological endpoints were characterized by using a battery of ecotoxicity tests to assess the soil hazard and the potential pollutant bioavailability. Effect on species representative of bacteria (i.e. dehydrogenase inhibition activity in Arthrobacter Globiformis), mesaufauna (i.e. Growth and reproduction test of the nematodes), macrofauna (i.e. earthworms behavior and acute toxicity test) and higher plants (i.e. oats and turnip) were studied. The accumulation of HAP, hydrocarbons and metals were determined ex situ in earthworms. In addition, the aquatic ecotoxicity of the hydrosoluble fraction of pollutant were investigated on freshwater microalgae and microinvertebrate. Finally, in situ ecological criteria (i.e. plant inventory) and pollutant transfer in plants were investigated on each site. The poster will present the proposed approach as well as the first results and their interpretation regarding the categorization of the investigated sites.
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Submitted on : Wednesday, June 30, 2021 - 11:04:49 AM
Last modification on : Thursday, October 28, 2021 - 4:32:10 PM


  • HAL Id : ineris-03274522, version 1


Nicolas Manier, Delphine Boulant-Cirederf, Olivier Faure, Jean-François Nau, Flora Druez, et al.. Integrated Biological Approach to Characterize and Discriminate Contaminated Wasteland in the Context of Its Requalification to an Ecological Use. 31. SETAC Europe annual meeting, Jun 2021, Online, Belgium. ⟨ineris-03274522⟩



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