Autologous induced pluripotent stem cell-derived four-organ-chip

Anja Patricia Ramme , Leopold Koenig , Tobias Hasenberg , Christine Schwenk , Corinna Magauer , Daniel Faust , Alexandra K. Lorenz , Anna-Catharina Krebs , Christopher Drewell (1) , Kerstin Schirrmann (2) , Alexandra Vladetic (3) , Grace-Chiaen Lin (3) , Stephan Pabinger (3) , Winfried Neuhaus (3) , Frédéric Y. Bois (4) , Roland Lauster (1) , Uwe Marx , Eva-Maria Dehne

1 TU - Technical University of Berlin / Technische Universität Berlin
2 University of Manchester [Manchester]
3 AIT Austrian Institute of Technology GmbH [Tulln, Austria]
4 INERIS - Institut National de l'Environnement Industriel et des Risques

Microphysiological systems play a pivotal role in progressing toward a global paradigm shift in drug development. Here, we designed a four-organ-chip interconnecting miniaturized human intestine, liver, brain and kidney equivalents. All four organ models were predifferentiated from induced pluripotent stem cells from the same healthy donor and integrated into the microphysiological system. The coculture of the four autologous tissue models in one common medium deprived of tissue specific growth factors was successful over 14-days. Although there were no added growth factors present in the coculture medium, the intestine, liver and neuronal model maintained defined marker expression. Only the renal model was overgrown by coexisting cells and did not further differentiate. This model platform will pave the way for autologous coculture cross-talk assays, disease induction and subsequent drug testing.

Keywords: DIFFERENTIATION; FOUR-ORGAN-CHIP; INDUCED PLURIPOTENT STEM CELLS; MICROPHYSIOLOGICAL SYSTEM; MULTI-ORGAN-CHIP

Domains: Toxicology

Anja Patricia Ramme, Leopold Koenig, Tobias Hasenberg, Christine Schwenk, Corinna Magauer, et al.. Autologous induced pluripotent stem cell-derived four-organ-chip. Future Science OA, 2019, pp.art. FSO413. ⟨10.2144/fsoa-2019-0065⟩. ⟨ineris-03319075⟩